摘要
目的:探讨免疫印迹技术对幽门螺杆菌的检测及分型诊断的应用价值。方法:应用免疫印迹技术检测58例上消化道疾病的患者和20例无症状健康志愿者血清中的幽门螺杆菌空泡毒素(VacA)、细胞毒素(CagA)和尿素酶(Ureas)等Hp抗体。根据Hp抗体的表达情况将Hp感染分为Ⅰ型和Ⅱ型,并结合^(14)C呼气试验和胃镜检查进行分析。结果:慢性胃炎组、消化性溃疡组、胃癌组的HpⅠ型阳性率分别为66.7%、89.5%、100%,与对照组(25%)的差别有统计学意义。慢性胃炎组、消化性溃疡组、胃癌组的HpⅡ型阳性率分别为20%、2.6%、0,慢性胃炎组HpⅡ型阳性率与对照组的差别无统计学意义,消化性溃疡组、胃癌组HpⅡ型阳性率与对照组的差别有统计学意义。并且发现随着病变恶性严重程度的增加,cagA/VacA的表达呈上升的趋势,尤其是VacA的表达,在慢性胃炎组、消化性溃疡组、胃癌组的阳性率分别为53.3%、81.6%、100%。结论:免疫印迹技术可应用于对Hp感染分型诊断,为临床治疗及判断预后提供一定依据。
Objective Investigate the applied value of detecting and typing Helicobacter pylori （Hp） by immunoblot assay. Methods The VacA, CagA, Ureas antibodies in serum for Hp of 56 patients who have disease of upper digestive tract and 20 health volunteers without symptoms in the control group were detected by immunoblot assay. Hp is divided into two subtypes base on those antibodies. And we analyse the Hp infection with those subtypes, the gastroscopy diagnosed and the 14C-Uneabreath test. Results Hp Ⅰ positive incidence of the chronic gastritis group, peptic ulcer group and gastric cancer group is 66. 7% ,89.5%, 100% respectively is statistically significant comparing with control group＇ s（25% ）. Hp Ⅱ positive incidence of the chronic gastritis group, peptic ulcer group and gastric cancer group is 20% , 2.6% ,0 respectively, The chronic gastritis group＇s incidence is not statistically significant comparing with control group＇ s, the other groups ＇ s incidence is statistically significant comparing with control group＇ s. And we found that, with the lesion getting worse the positive incidence of VacA/ CagA antibody will be rose, especially the positive incidence of the VacA antibody in the chronic gastritis group （53.3%）, peptic ulcer group （81.6%）, and gastric cancer group （ 100% ）. Conclusion Immunoblot assay can be used in detecting and typing Hp and it could provide some basis for clinical treatment and judging the prognosis.
出处
《实用医技杂志》
2007年第32期4393-4395,共3页
Journal of Practical Medical Techniques
关键词
免疫印迹技术
幽门螺杆菌
分型
immunoblot assay
Helicobacter pylori
subtypes
