摘要
目的:探讨外源性hCG调控先天性尿道下裂小鼠阴茎组织EGF的作用。方法:采用昆明种小鼠建立尿道下裂动物模型。选用出生3周龄的正常雄性小鼠和先天性尿道下裂雄性小鼠各10只,腹腔内注射等量生理盐水作为对照;40只尿道下裂小鼠均分为4组,腹腔内分别注射hCG50、100、150、200IU,1次/d,共7d。酶联免疫吸附法定量检测各组小鼠阴茎组织和血清中EGF的浓度。结果:先天性尿道下裂小鼠阴茎组织EGF的浓度[(47.54±18.61)pg/ml]明显低于正常组[(84.10±21.87)pg/ml](P<0.05);采用hCG外源性干预后,阴茎组织中EGF的浓度随hCG剂量的增加而升高,分别为50IU剂量组(59.57±22.16)pg/ml、100IU剂量组(57.97±9.59)pg/ml、150IU剂量组(73.02±31.35)pg/ml和200IU剂量组(92.45±35.74)pg/ml。150IU和200IU剂量组EGF的浓度与对照组小鼠差异无显著性(P>0.05)。结论:小鼠阴茎组织EGF浓度降低可能与尿道下裂的发生存在一定关系。一定剂量范围内的外源性hCG,可使先天性尿道下裂小鼠阴茎组织EGF浓度增加。
Objective ： To investigate the impact of hCG on the epidermal growth factor （EGF） in the phallic tissues of hypospadiac mice. Methods： The Kunming mouse model of congenital hypospadias was established. As controls, 10 three-week-old normal male mice and 10 3-week-old congenital hypospadiac mice were injected with isometric sodium chloride intraperitoneally. Another 40 mice with hypospadias were divided into 4 groups and injected intraperitoneally with 50 IU, 100 IU, 150 IU and 200 IU of hCG respectively per day for 7 days. Then the concentration of ECG in the phallus and in the serum were assayed by ELISA. Results ： The EGF level in the phallus of the hypospadiac mice was much lower than that of the controls statistically （ P 〈 0.05 ）. After the injection of exoge- nous hCG, the concentration of ECG increased with the dose of hCG, 50 IU （59.57±22.16） pg/ml, 100 IU （57.97±.59） pg/ml, 150 IU （73.02±31.35 ） pg/ml and 200 IU （92.45±35.74 ） pg/ml. The concentration of ECG showed no significant difference be-tween the control group and the 150 IU and 200 IU groups （P 〉 0.05 ）. Conclusion： Decreased concentration of EGF in the mouse phallus may be associated with the pathogeny of hypospadias. A proper dose of exogenous hCG may increase the concentration of EGF in the mouse phallus.
出处
《中华男科学杂志》
CAS
CSCD
2007年第12期1072-1074,共3页
National Journal of Andrology
基金
山西省自然科学基金资助(991079)
