摘要
用单扫示波极谱法研究了青藤碱和黄芩甙的电化学行为，青藤碱在０．１ｍｏｌ／ＬＮａ２Ｂ４Ｏ７中有两个还原峰，峰Ｐ１和Ｐ２的电位分别为－１．４５Ｖ和－１．６７Ｖ（μｓ．ＳＣＥ），峰Ｐ１的峰电流与浓度在０．０６～１．８ｍｇ／Ｌ和２．０～３４．６ｍｇ／Ｌ范围内呈线性关系，检测限为０．０２ｍｇ／Ｌ。在０．０５ｍｏｌ／Ｌ柠檬酸钠＋０．２ｍｏｌ／Ｌ ＨＣｌ底液中，黄芩甙于－１．１４Ｖ（Ｐ３）和－１．３２Ｖ（Ｐ４）（μｓ．ＳＣＥ）处产生两还原峰。利用峰Ｐ３可测定黄芬甙，线性范围为０．１６～０．６４ｍｇ／Ｌ和０．７２～２４ｍｇ／Ｌ，检测限为０．０８ｍｇ／Ｌ。用本法测定中药青风藤中总生物碱和中药黄芩甙的含量，结果令人满意。讨论了青藤碱和黄芩甙对超氧自由基（Ｏ２）的清除作用。
The electrochemical behaviour of baicalin and sinomenine is studied by single sweep oscillopolarography. Sinomenine shows two reduction waves in 0. 1 mol/L Na,B.O, base solution and the peak potentials are - 1. 45 V and - 1. 67 V (vs. SCE) respectively. The peak height of wave P, is proportional to the concentration in the range of 0. 06 - 1. 8 mg/L and 2. 0-34. 6 mg/L respectively, and the detection limit is 0. 02 mg/L. In the base solution of 0. 05 mol/L trisodium citrate +0. 2 mol/L HCl, baicalin shows two reduction waves with the peak potentials - 1. 14 V(P3) and 1. 32 V(P4) (vs. SCE), respectively. The peak height of the first wave (P3) is proportional to the concentration in the range of 0. 16- 0. 64 mg/L and 0. 72-24 mg/L respectively, and the detection limit is 0. 08 mg/L. It is em- ployed for the determination of totol alkaloids in sinomenium acutum (Thumb. ) Rehd. et Wils and baicalin in Scutellaria Daicalensis Georgi. In addition, it is discus8ed that sinomenine and baicalin can scavenge superoxide anion radical produced by the auto-oxidation of pyrogallol.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
1997年第5期551-554,共4页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金
中国科学院长春应用化学研究所电分析化学开放研究实验室基金资助项目
