摘要
在碱性介质中，黄芩甙对铁氰化钾氧化鲁米诺产生的化学发光有较强的增敏作用，据此建立了流动注射化学发光测定黄芩甙的新方法．详细研究了各因素对化学发光反应的影响，在选定的最佳实验条件下，黄芩甙的浓度在5．0×10^-8～3．0×10^-5g／mL范围内与发光强度呈良好的线性关系．对浓度为1．0×10^-5g／mL的黄芩甙进行11次平行测定，其相对标准偏差为3．86％．根据IUPAC的建议，计算得到的检出限（3σ）为1．0×10^-9g／mL．将本法用于合成样品及尿样中黄芩甙的测定，获得满意结果。
It was found that the light emission produced by the oxidation of luminol by potassium ferricyahide in the basic medium was enhanced by baicalin. The optimum conditions for this chemiluminescent reaction were studied in detail by a flow injection system. A new, simple and rapid method has been deveoped under the optimum conditions for determination of baicalin. This method has the advantages of high sensitivity, good reproducibility and low detection limit. In the optimum conditions, CL intensiti proportional to concentrations of the baicalin in the 5.0 ×10^-8 - 3.0 × 10^-6 g/mL range. The limit teetion is 1.0× 10^-9 g/mL for baiealin. The method has been applied to the determination of baicalin in the synthetical samples and biological fluids with satisfactory results.
出处
《西南师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2006年第3期85-88,共4页
Journal of Southwest China Normal University(Natural Science Edition)
基金
国家科技攻关西部引导项目(2003BA869C)
教育部"春晖计划"项目(2004722)
重庆市自然科学基金重点项目(2004BA7019)资助
关键词
化学发光
流动注射
黄芩甙
药物分析
chemiluminescence flow injection analysis
baicalin pharmaceutical analysis
