摘要
本研究为探讨甾体避孕药能否对卵泡细胞产生直接的抑制作用。实验用猕猴13只,分单给18甲、单给PMSG、合并给以上二药和空白对照四组。给药前后对动物活体抽取卵泡液、外周血和卵巢静脉血,用放射免疫法测定其中的E_2、P_0、A和18甲水平。结果提示甾体避孕药18甲似能直接进入卵泡液,并影响卵泡颗粒细胞甾体的生成。
The effect of norgestrel (NOG) on the ovarian follicles were studied in 13 regularly menstruating rhesus monkeys. The animals were divided into 4 groups: NOG treated group (2), PMSG stimulated + NOG treated group (4), PMSG stimulated group (2) and control group (5). PMSG 200 IU/day were injected from the 5th me day for 5 days. NOG 0.5 mg/kg of body weight was injected 24 hours before laparotomy. Estradiol (Ea), androstenedione (A) and progesterone (P.) levels were measured in the peripheral blood, ovarian venous blood and follicular fluid in all groups. Concentrations of NOG were measured only in the NOG treated and PMSG + NOG treated group. In one of the NOG treated monkeys the E2 levels were decreased in the ovarian venous blood and follicular fluid, while the P. was decreased only in the follicular fluid. No significant differences were found in the steroid hormone levels between the PMSG and PMSG+NOG treated group. High levels of NOG were detected in the peripheral blood and ovarian venous blood in all treated monkeys, but were detected only in 2 out of 5 samples of follicular fluid, which were at very high levels (62.35 ng/ml and 45.71 ng/ml), about 2.5-7 times higher than their own ovarian venous blood level and 2.5 times higher than the peripheral blood level. Transport of steroid contraceptives into the follicular fluid and the possible direct effect on the function of granular cells and the maturation of oocyte need further investigations.
出处
《动物学报》
SCIE
CAS
CSCD
1989年第1期53-57,共5页
ACTA ZOOLOGICA SINICA
关键词
18甲炔诺酮
猕猴
甾体激素
卵泡液
norgestrel, rhesus monkeys, steroid hormone, follicular fluid
