摘要
采用羊膜双池培养系统观察猪卵巢颗粒细胞（Ｇ－Ｃ）和内泡膜细胞（Ｔ－Ｃ）在甾体激素分泌中的相互作用，并与纤维膜双池相比较。分别将Ｇ－Ｃ和Ｔ－Ｃ种在羊膜两侧，内池为Ｇ－Ｃ，外池为Ｔ－Ｃ；经４８小时培养后收集内、外地培养液，测定雌二醇（Ｅ２）、孕酮（Ｐ）和睾酮（Ｔ）含量。结果表明：在羊膜双池系统中生长的Ｇ－Ｃ和Ｔ－Ｃ比纤维膜双池培养的细胞保持了更活跃的甾体激素分泌功能。外地Ｔ－Ｃ分泌的更多Ｔ透过羊膜到达内地，作为芳香化酶底物，使Ｇ－Ｃ合成更多Ｅ２（可达２４３５ｐｍｏｌ／Ｌ）；Ｇ－Ｃ的Ｐ（５２５μｍｏｌ／ｕ和Ｔ（１０．２μｍｏｌ／Ｌ）产量也高于纤维膜双池。本研究还观察了促性腺激素对羊膜双池培养Ｇ－Ｃ和Ｔ－Ｃ分泌功能的作用。羊膜双池培养模拟了在体的两种卵巢细胞在甾体激素合成和分泌过程中的相互作用，是研究Ｇ－Ｃ和Ｔ－Ｃ的旁分泌调节较理想的模型。
a Lian Family Planning Research Institute Objectives:To study the interaction between granulosa andthecal cells cultured in an amniondual cham-ber,in comparison with the dual chamber of Matrigel.Design:An in vitro culture study.setting:Endocrinology laboratory in National Research Institute for Family Planning. Methods:Adual chamber culture system was prepared with amnion membrane from human term placenta.The isolated porcine granulosa cells (G-C)and thecal eclls(T-C)were grown on both sides of the amnion membrane,with G-Cin the inner chamber and the T-C in the outer chmber.The concentra- tions of estradiol(E2),progesterone (P) and testosterone(T)in the culture medium were measured by radioimmunoassay(RIA).Matrigel dual chamber system was set up for comparison.Results:The growth of both granulosa and thecal cells and their steroidogenesis function in amnion dual chamber were more active than in the Matrigel dual chamber system:(1) more T produced by T-C passed into the inner chamber through the amnion membrane,and used as the substrate of arornatization by G-C,so that Tnore E2 was produced(up to 2 435 pmol/L);(2)the production of P(525μmol/L) and T(10.2μmol/L)by G-C was also higher than those in the Matrigel dual chamber system,The go-nadotropins added to the culture medium stimalated the steroidogenic function of P by G-C and T-C.Conclusions:The dual chamber system made of amnion membrane has better effect than the Ma-trigel dual chamber system.can be used as an in vitro model for studying paracrine regulatory interac-tion between granulosa and thecal cells.
出处
《生殖医学杂志》
CAS
1996年第1期16-20,共5页
Journal of Reproductive Medicine
关键词
粒层细胞
卵泡膜细胞
羊膜
双池培养
甾类
Granulosa cells Theca cells Amnion Dualchamber system Steroid Paracrine
