摘要
大豆异黄酮的测定可采用单波长法、三波长法和液相色谱法.单波长法(y=7.431 7x-0.055 6,R2=0.999 8)和三波长法(y=32.432x+0.048 8,R2=0.998 1)用于总异黄酮测定,操作简便.高效液相色谱法(染料木甙,y=1 828.6x-96.467,R2=0.996 4;大豆甙,y=1 795.4x-420.67,R2=0.995 3)用于单个异黄酮成分的测定,操作较复杂.精密度和准确度依次为:液相色谱法>三波长法>单波长法.
Soybean isoflavones were determined with a single-wave-length method, a three-wave-length method and HPLC. Single-wave-length method （ y = 7.431 7x - 0.055 6, R^2 = 0.999 8） and three-wave-length method （y = 32.432x ＋ 0.048 8, R^2 = 0.998 1） were used conveniently for total isoflavones. HPLC （genistin, y = 1 828.6x - 96.467, R^2 = 0.996 4; daidzin, y = 1 795.4x - 420.67, R^2 = 0.995 3） was used for single isoflavone, which was complicated. Accuracy and precision of the three methods in a descending order is HPLC, three-wave-length method, and single-wave-length method.
出处
《上海大学学报（自然科学版）》
CAS
CSCD
北大核心
2007年第6期741-745,共5页
Journal of Shanghai University:Natural Science Edition
