摘要
应用Hungate厌氧技术,从大庆油田聚合物配注站的母液罐中分离到一株水溶性超高分子量聚丙烯酰胺(HPAM)的降解菌株I8,该菌株为短杆状,G-,黑色圆形菌落,最适温度为38℃,最佳pH值为7.8,具有硫酸盐还原功能,产H2S气体,兼性厌氧.研究表明,该菌株能以HPAM为唯一碳源,降解侧链,部分官能团发生改变;浓度为600mg/L时,20d菌株生物降解率为63.17%,其溶液粘度下降效果显著.16S rDNA序列与Enterobacter cloacae(ECL251469)的相似性为98%,通过形态、生理生化、G+C含量以及16S rDNA序列鉴定,初步鉴定可能为肠杆菌属的一个新种,暂时命名为Enterobacter HPAM Degraded Bacteria I8,I8菌株的分离为HPAM的生物降解提供了新的微生物资源.图4,参14.
A hydrolyzed polyacrylamide （HPAM）-degrading bacteria strain I8 was isolated from curing pot in HPAM distribution station of Daqing Oil Field using Hungate anaerobic technique. It was gram negative, short bacillus, with an optimum at pH 7.8, with an optimum growth temperature of 38℃, intersexual anaerobic bacteria, black circular colony, which can reduce sulfate to H：S. Results showed that the bacteria can use HPAM as only carbon source, making HPAM polymer surface structure changed, and hydrolyzing amide to carboxyl group by the molecule chain amido hydrolysis, degrading the side-chain and changing some function group. The HPAM solution density is 600 mg/L, 20 days strain biodegradation rate is 63.17%, resulting in obviously decreased viscosity. Systemic anagenesis indicated that the resemblance level between 16S rDNA sequence of the I8 strain and Enterobacter cloacae （ECL251469） is 98%, combined the strain morphology, physiologically, biochemically, G＋C content and 16S rDNA sequence, the isolate was identified as a novel species belongs to Enterobacter, temporarily named Enterobacter HPAM Degraded Bacteria I8. The I8 strain isolated provid a new microorganism resources for the polyacrylamide biodegradation. 4figs., 14refs.
出处
《湖南科技大学学报（自然科学版）》
CAS
北大核心
2007年第4期122-125,共4页
Journal of Hunan University of Science And Technology：Natural Science Edition
基金
国家自然科学基金重大国际合作项目(50521140075)
