摘要
目的建立丹蛭合剂(丹参、钩藤、首乌藤、桑寄生等)的质量标准。方法采用TLC法鉴别处方中丹参、首乌藤和钩藤,采用HPLC法测定丹酚酸B的含量。结果TLC色谱能特异地鉴别丹参、钩藤和首乌藤。丹酚酸B进样量在252～5040ng范围内线性关系良好,回归系数r=0.9999,平均回收率为100.7%,RSD为0.73%(n=6)。结论该方法专属性强,简便可行,重现性好,能有效地控制丹蛭合剂的内在质量。
Objective To establish the quality standard of ＂Danzhi Mixture＂. Methods Radix et Rhizoma salviae mihiorrhizae, Ramulus uncariae cure uncis, and Caulis polygoni nultiIlori were identified by TLC. The content of Salvianolic acid B was determined by HPLC. Results Radix et Rhizoma salviae mihiorrhizae, Ramulus uncariae cure uncis, and Caulis polygoni nultiflori could be determined by TLC. Salvianolic acid B showed good linear relationship in the rages of 252-5040 ng, r=0.999 9. Recovery was 100.7%, RSD=0.73% （n=6）. Conclusion The methods are sensitive, accurate and reproducible.
出处
《上海中医药杂志》
北大核心
2007年第12期60-62,共3页
Shanghai Journal of Traditional Chinese Medicine
基金
国家自然科学基金资助项目(30572381)
关键词
丹蛭合剂
丹酚酸B
首乌藤
钩藤
HPLC
＂Danzhi Mixture＂
salvianolic acid B
Radix et Rbizoma salviae mihiorrbizae
Ramulus uncariae cure uneis
Caulis polygoni nultiNori
HPLC
