摘要
目的:建立凝聚态β-淀粉样蛋白(Aβ1-40)诱导的模拟阿尔茨海默病(Alzheimer’sDisease,AD)的动物模型,观察其学习记忆及病理性改变。方法:将凝聚态Aβ1-40注射入大鼠双侧海马,大鼠跳台和Morris水迷宫试验检测大鼠的学习记忆能力,HE和银染检测病理变化。结果:海马内注射凝聚态Aβ1-40可引起大鼠学习记忆能力下降,海马注射区可见神经元丢失、胶质细胞增生及神经原纤维缠结。结论:凝聚态Aβ1-40海马内注射具有明确的在体神经毒性作用,可以模拟AD的学习记忆障碍、神经元损伤等行为学和病理学方面的特征。
Objective：To establish a rat model with Alzheimer＇s disease （AD） induced by incubated Aβ1-40 and study the changes of learning and memory ability and pathology. Methods：Incubated Aβ1-40 was microinjected into the bilateral hippocampus of each rat. Jumplag test and Morris water maze test was used to evaluate rat learning and memory function. The pathological changes were observed by haematoxylin-eosin （HE） and silver staining. Results：The capability of learning and memory was impaired significantly after Aβ1-40 injection. Neuron death,glial reaction and neurofibrillary tangles were found in or around the injection sites. Conclusion： These results suggest that the aggregated and deposition of Aβ1-40 in the hippocampus was related to the impairment of learning and memory, and the neuronal degeneration in the rats which showed similar behavior and pathological characterizations of AD. It could be used as an animal model for the prevention and cure of AD.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2007年第12期1373-1376,I0001,共5页
Journal of Nanjing Medical University(Natural Sciences)
