摘要
目的：研究烧伤后肠道一氧化氮（ＮＯ）及一氧化氮合酶（ＮＯＳ）的变化规律。方法：采用３０％总体表面积（ＴＢＳＡ）Ⅲ度烧伤大鼠模型，分别检测了肠组织中ＮＯ含量及原生型ＮＯＳ（ｃＮＯＳ）和诱导型ＮＯＳ（ｉＮ－ＯＳ）的活性，并分析了ＮＯ的变化规律及其同两型ＮＯＳ之间的关系。结果：烧伤后肠组织中ｉＮＯＳ活性大幅上升，与ＮＯ的变化趋势一致，二者呈显著正相关（ｒ＝０．９５，Ｐ＜０．０１）而ｃＮＯＳ活性呈下降趋势，与ＮＯ相关不显著（ｒ＝－０．６１，Ｐ＜０．０５）。结论：烧伤后肠组织中ＮＯ的变化主要受ｉＮＯＳ活性影响，ｉＮＯＳ活性上升，ｃＮＯＳ活性下降可能与烧伤后肠道某些病理变化密切相关。
Objective: To explore the regularity of the changes of nitric oxide synthase (NOS) and nitric oxide (NO) in the small intestine postburn. Methods: The Wistar rats with 30% TBSA full thickness skin burns were employed to determine the activity of inducible NOS (iNOS) and constitute NOS (cNOS) and the amount of NO in the small intestine. Results: The iNOS activity and the amount of NO were significantly increased postburn and there was a positive correlation between them (r=0.95, P<0.01). However, the cNOS activity was remarkably decreased and it was not positively correlated with amount of NO (r=-0.61, P>0.05). Conclusion: The changes of NO in the small intestine are mainly affected by iNOS activity and the changes of the activity of iNOS and cNOS are related to some pathological changes in the small intestine postburn.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
1997年第3期191-193,共3页
Journal of Third Military Medical University
基金
全军"九五"攻关项目
关键词
一氧化氮
一氧化氮合酶
烧伤
nitric oxide
nitric oxide synthase
intestine
burns
rat
