摘要
目的:筛选和优化环孢素A-聚氰基丙烯酸酯纳米粒处方工艺,并初步考察了纳米粒的相关理化性质。方法:反相高效液相色谱测定环孢素含量;聚氰基丙烯酸酯为载体材料,均匀设计法对乳化聚合技术制备载药纳米粒中油/水比例,乳化刑、蒸发温度和乳化时间进行优化:透射电镜对纳米粒特性和形态进行了鉴定分析。结果:以水:溶剂为5:1作溶剂,0.1%泊洛沙姆为增溶剂,温度为50℃下所制备的环孢素纳米粒分布均匀集中,呈单峰,粒子呈圆形,平均粒径为(39.75±0.50)nm,回收率为(92.21±2.06)%,包封率为(99.57±0.04)%,载药量为(15.33±0.01)%。结论:优化处方工艺制备的环孢素A-景氰基丙烯酸酯纳米微粒有良好稳定性和分散性。
Objective： To select the best preparation technique of intestines dissolved materials, polycyanoacrylate nanoparticles loaded cyclosporine A. Methods： The cyclosporine A nanoparticles were prepared by the emulsified- polymerization method. The contents of cyclosporine A were determined by RP-HPLC. The preparation technique and prescription of nanoparticls were optimized by uniformity design regulation, Results： The nanoparticles had a small particle size and the high recovery and envelopment rate when the ratio of water and solvent phase was 5：1, using 0.1% pluronic F68 as solubilizing agent. The average particle size was 39,75 ± 0,50 nm, the yield was 92.21 ± 2,06%, the drug entrapped efficiency was 99.57 ±0.04% and the drug loading was 1.53 ± 0.01%. Conclusion： The optimal prescription and preparation technique of cyclosporine-loaded polycyanoacrylate nanoparticles were obtained.
出处
《抗感染药学》
2007年第4期154-157,186,共5页
Anti-infection Pharmacy
基金
江苏省卫生厅基金资助(编号:H2006030)
苏州大学医学发展基金资助(编号:Ee132503)
关键词
环孢素A
纳米粒
制备
均匀设计
Cyclosporine A
nanoparticles
preparation
uniformity design
