摘要
应用四甲基偶氮唑盐（ＭＴＴ）法检测了３１例未经治疗的囊虫病患者外周血单个核细胞（ＰＢＭＣ）产生ＩＬ－２水平，同时用ＥＬＩＳＡ双抗体夹心法测定其血清中ｓＩＬ－２Ｒ水平，以３０例健康人作对照。实验结果显示，囊虫病患者ＰＢＭＣ产生ＩＬ－２水平明显低于正常人（Ｐ＜０．０５），而患者血清中ｓＩＬ－２Ｒ水平明显高于正常人（Ｐ＜０．０１）。本实验提示囊虫病患者外周血中低水平的ＩＬ－２及血清中高水平的ｓＩＬ－２Ｒ可能与患者细胞免疫功能低下有关。
To investigate the changes and effects of cytokines in patients with cysticercosis,3 (4,5 dimethylthiazol 2 yl) 2,5 di phenyltetrazolium bromide(MTT) was used to assay the levels of IL 2 in culture supernatant of peripheral blood mononoclear cells(PBMC) in patients with cysticercosis and healthy controls. The levels of soluble IL 2 receptor in sera were also assayed in both groups with ELISA. The results showed that the levels of IL 2 in culture supernatant from PBMC of patient group were significantly lower than that of control group( P <0.05),but the level of sIL 2R in sera from patients was significantly higher than that in control group( P <0.01). These results suggested that the lower level of IL 2 in culture supernatant of PBMC in patients with cysticercosis and the higher level of sIL 2R in sera from patients might be related to weak cellular immune function of patients.
出处
《中国寄生虫病防治杂志》
CSCD
1997年第2期97-99,共3页
Chinese Journal of Parasitic Disease Control
