摘要
从土样中分离获得的链霉菌ＳＯ１菌株在产酶培养基中，２８℃振荡培养７天，其胞外几丁质酶活力可达１８０９ｕ／ｍｌ。实验表明，不同碳、氮源及诱导物对ＳＯ１菌株产几丁质酶有不同的影响，以酵母粉对产酶的促进作用最为明显，比对照提高了７５．２％。部分脱乙酰几丁质、胶体几丁质及几丁质均可作为ＳＯ１菌株产几丁质酶的诱导物，其中以几丁质诱导产酶的效果最好。
The chitinase activity of Strptomyces sp.SO1 which was obtained from soil could reach 1809u/ml after SO1 was cultivated in the shaker for 7 days at the temperature of 28℃.It was showed that different inducers,carbon and nitrogen sources had different impacts on SO1 strain.Yeast powder was the most distinctive factor,which enhanced the enzyme activity by 75.2% compared with the comparison.Partial deacetyl chitin,collodial chitin could be used as the inducers of SO1 strain for the production of chitinase.Among them,chitin was the best inducer.
出处
《天津农业科学》
CAS
1997年第3期1-4,共4页
Tianjin Agricultural Sciences
关键词
链霉菌
几丁质酶
筛选
培养基
Strptomyces sp
SO1 strain
chitinase
culture condition
