摘要
目的测定替加环素等15种抗菌药物对我院2004年和2005年临床分离的414株革兰阴性菌和革兰阳性需氧菌的体外抗菌活性。方法采用微量肉汤稀释法测定替加环素等15种抗菌药物对所测菌株的MIC,数据分析采用WHONET5.3软件。结果MRSA对替加环素、利奈唑胺及万古霉素的敏感率均为100%,替加环素对MRSA的MIC90是所测抗菌药物中最低者;万古霉素耐药肠球菌（VRE）对替加环素及利奈唑胺敏感率均为100%,替加环素对所有肠球菌的MIC90分别是利奈唑胺和万古霉素的MIC90的1/8和1/16;替加环素对青霉素中介肺炎链球菌（PISP）的MIC90为0.5mg/L,对青霉素耐药肺炎链球菌（PRSP）的MIC范围是0.25~1mg/L,其他抗菌药物对PISP和PRSP的MIC90是替加环素的1~32倍;替加环素对亚胺培南耐药的鲍曼不动杆菌的MIC范围是其他抗菌药物的1/2~1/64;对铜绿假单胞菌的MIC90是32mg/L;产ESBLs大肠埃希菌和肺炎克雷伯菌对替加环素、美罗培南和亚胺培南敏感率均为100%。结论替加环素对铜绿假单胞菌的抗菌活性较差,对其他需氧革兰阴性杆菌有较好的体外抗菌活性;对需氧革兰阳性球菌的抗菌活性最强。
Objective To investigate antimicrobial activity of tigecycline and 14 comparators against 414 gram-negative and gram-positive pathogens isolated in 2004 and 2005 in Peking Union Medical College Hospital. Methods Broth microdilution method was used to determine the minimum inhibitory concentrations （MICs） of tigecycline and other 14 antibiotics against these strains. WH（）NET 5. 3 software was used to analyze the data. Results All oxacillin-resistant Staphylococcus aureus （MRSA） were susceptible to tigecycline, linezolid and vancomycin. The MIC90s of other antimicrobial agents are 1-fold to 256- fold higher than that of tigecycline. Tigecycline and linezolid showed the same susceptibility rate of 100% to vancomycin-resistant Enterococcus （VRE）. MIC90 values of linezolid and vancomycin were 8-fold and 16-fold higher than that of tigecycline. MIC90 value of tigecycline was 0.5 mg/L against penicillin-intermediate S. pneumoniae （PISP） and 0.25-1 mg/L against penicillin-resistant S. pneumoniae （PRSP）. MIC90 values of other antimicrobial agents were from 1-fold to 32-fold higher that of tigecycline for PISP and PRSP. MIC range of tigecycline against imipenem-resistant A. baumannii was 1-fold to 64-fold lower than that of other comparators. MIC90 value of tigecycline was 32 mg/L against P. aeruginosa. ESBLs-producing K. pneumoniae and E. coli were 100% susceptible to tigecycline, imipenem and meropenem. Conclusions P. aeruginosa is the organism least susceptible to tigecycline. Tigecycline is the most active agent against gram-positive cocci and showed good in vitro activity against gram-negative clinical isolates.
出处
《中国感染与化疗杂志》
CAS
2007年第6期448-455,共8页
Chinese Journal of Infection and Chemotherapy
关键词
替加环素
微量肉汤稀释法
耐药性
Tigecycline
Broth microdilution method
Resistance
