摘要
云南红豆杉培养细胞系的建立罗建平牛炳韬贾敬芬郑光植１（兰州大学生物系兰州７３００００）（中国科学院昆明植物研究所昆明６５０２０４）１紫杉醇（Ｔａｘｏｌ）最初是从红豆杉属植物短叶红豆杉（Ｔａｘｕｓｂｒｅｖｉｆｏｌｉａ）树皮中分离出的一种二萜类化合物［...
Callus culture system was established from Taxus yunnanensis cheng et L.K.Fu.using different explants including young stems and needles on MS and B 5 medium.Young stems were easier than young needles for initiating callus.Callus derived from stem segments displayed better growth on defined media.B 5 medium was more suitable than MS medium for callus growth,although lower frequency of callus formation was observed.The callus growth was evidently influenced by different 2,4 D concentrations in combination with kinetin or different organic additives.The best growth rate was 0.283g·(L·d) -1 ,which was obtained when calli were maintained on subculture medium supplemented with 2mg/L 2,4 D,0.5mg/L kinetin and 0.1% casein hydrolysate.The growth curve of T.yunnanensis callus exhibited that the optimum period for callus subculture was 35 days and the maximum increase in biomass was 3.32 times of the initial dry weight during the stationary phase.A lag phase was apparent between day 0 and the fifteenth day.The dry weight doubling time was about 15 days during the exponential phase. T.yunnanensis cell cultures were able to synthesize taxol by high performance liquid chromato graphy (HPLC) analysis and the highest taxol content was 0.029% DW. The comparsion between cell growth rate and taxol content showed that there was extreme variability among different established callus lines and revealed that there was an inverse relationship between cell growth rate and taxol content ( r =-0.6375) to some extent.
出处
《生物工程学报》
CAS
CSCD
北大核心
1997年第3期326-330,共5页
Chinese Journal of Biotechnology
基金
国家自然科学基金
关键词
红豆杉
细胞系
细胞培养
Taxus yunnanensis ,tissue culture,taxol
