摘要
目的观察小型猪腮腺萎缩后混合唾液流率的变化以及对口腔主要致病菌的影响。方法10只小型猪分成两组,每组5只,其中一组双侧腮腺注入甲紫致腺体萎缩,另5只作为健康对照组。分别在萎缩后第12、24个月收集混合唾液并观察唾液流率的变化;同时检测小型猪龈下菌斑和混合唾液中口腔主要致病菌数量并与健康对照组比较。结果腮腺萎缩后第12个月混合唾液流率自(1.36±0.74)ml/min下降到(1.32±0.65)ml/min,第24个月唾液流率自(0.72±0.34)ml/min上升到(0.86±0.57)ml/min;龈下菌斑中的核梭杆菌、后牙龈下菌斑中厌氧菌总数、产黑菌数量均明显高于健康对照组;第12个月前牙和第24个月后牙的龈下菌斑中变形链球菌有短暂升高,而需氧菌总数、乳酸菌和放线菌数量无明显变化。唾液中除变形链球菌和产黑菌在腮腺萎缩早期有轻度上升外,其余细菌数量均无明显变化。结论双侧腮腺萎缩后明显降低口腔中唾液总量,导致牙菌斑中的致龋菌和牙周致病菌数量上升。
Objective To observe the effect of bilateral parotid gland atrophy on the whole saliva flow rate and the growth of main oral pathogens in different sites of oral cavity. Methods Ten healthy miniature pigs were divided into two groups. The parotid glands of test group （ n = 5） were bilaterally ablated by methyl violet. Another healthy five miniature pigs served as the control group. Whole saliva was collected and the whole saliva flow rate detected in both groups at 12 and 24 months respectively after parotid atrophy. The total numbers of oral main pathogens in the first molar, cuspid sub-gingival bacteria plaque and whole saliva were also detected. Results The whole saliva flow rate was significantly decreased at both 12 and 24 months respectively after atrophy of bilateral parotid gland in miniature pig. Pathogens including Streptococcus mutans, Porphyromonas gingivalis and Fusobacterium nucleatum in different sites oral cavity were increased after bilateral parotid gland atrophy. Conclusions Bilateral ablation of the parotid glands led to a significant decrease of whole saliva flow rate. The total numbers of main oral pathogens were increased in different sites of oral cavity.
出处
《中华口腔医学杂志》
CAS
CSCD
北大核心
2007年第12期737-740,共4页
Chinese Journal of Stomatology
基金
国家自然科学基金(30430690)
北京市自然科学基金(7042025)
关键词
腮腺
唾液分泌
细菌
动物
实验
Parotid gland
Salivation
Bacteria
Animals,labortory
