摘要
目的:探讨五倍子水提取物对牙龈卟啉菌(Porphyromonas gingivalisPg)膜泡(extracellular vesicles ECV)抑制牙周膜成纤维细胞生物活性的影响。方法:采用体外培养的人牙周膜成纤维细胞,用3H-TdR掺入方法,检测ECV对人牙周膜成纤维细胞DNA合成的影响,以及中药五倍子提取物对ECV这一生物活性的阻断作用。结果:细胞培养物中加入50μg/mLECV时,人牙周膜成纤维细胞DNA的合成受到明显抑制,与空白对照组相比P<0.01。当同时加入各浓度五倍子水提取物时其DNA合成量明显增加,与ECV组相比均(P<0.05),且随五倍子水提取物浓度升高,DNA的合成量也随之增高,并呈一定浓度依赖性。结论:适宜浓度的五倍子提取物可有效地阻断ECV的这种生物活性作用,从而可推测其对牙周病的发生、发展能起到一定的阻断作用。
AIM：To study the effect of chinese nutgall extract on biological activity of human periodontal ligament fibroblasts inhibited by Porphyromonas gingivalis membrane extracellular vesicles（ECV）.METHODS：3H-TdR incorporation method was used to examine the synthesis of DNA in cultured human periodontal ligament fibroblasts stimulated by ECV and blocking role of chinese nutgall extract on ECV.RESULTS：Compared with control group,the synthesis of DNA of cultured human periodontal ligament fibroblasts was inhibited significantly when 50 μg/mL of ECV was added（P〈0.01）;Compared with ECV group,the synthesis of DNA of cultured human periodontal ligament fibroblasts was increased significantly when chinese nutgall extract was added（P〈0.01） and its biological effect seemed concentration-dependent manner.CONCLUSION：Chinese nutgall extract of appropriate concentration may block effectively biological ability of ECV.Thus speculated that Chinese nutgall extract may block development of periodontal dieases.
出处
《牙体牙髓牙周病学杂志》
CAS
2007年第12期695-697,共3页
Chinese Journal of Conservative Dentistry
关键词
五倍子
牙龈卟啉菌
膜泡
牙周膜成纤维细胞
chinese nutgall
porphyromonas gingivalis
extraccellular vesicles
periodontal ligament fibroblasts
