摘要
生长调节物质对丹参叶片脱分化及根芽分化效应的研究结果表明：（１）０．５～５．０ｍｇ／Ｌ２，４－Ｄ、０．１～０．５ｍｇ／ＬＮＡＡ、０．５～１．５ｍｇ／ＬＩＢＡ或０．２～１．０ｍｇ／Ｌ４ＰＵ－３０诱导叶片的脱分化率高达９７％～１００％．（２）０．１～０．５ｍｇ／ＬＮＡＡ或０．５～２．０ｍｇ／ＩＢＡ诱导芽分化率为９３％～１００％；３种细胞分裂素中，４ＰＵ－３０对叶片芽分化最有效，６－ＢＡ和ＫＴ次之．（３）随２，４－Ｄ、ＮＡＡ和ＩＡＡ浓度增大，愈伤组织或再生苗的根分化率也增高．
The present investigation deals with the effect of growth regulating substances on the dedifferentiation and roots and buds differentiation in tissue culture of the blade of salvia miltiorrhiza Bunge.The results were that:(1)The frequency of the dedifferentiation induced by 2,4 D (0.5～5.0 mg/L),NAA (0.1～0.5 mg/L) ,IBA (0.5～1.5 mg/L)or 4PU 30 (0.2～1.0 mg/L) was 97%～100%. (2)The frequency of buds differentiation induced by NAA (0.1～0.5 mg/L), or IBA (0.5～2.0 mg/L) was 93%～100%, among the three cytokinins tested, 4PU 30 was superior to 6 BA and KT in the blade budding. (3)The frequency roots differentiation induced from the callus or from the regeneration buds was gone up with the increasing concentration of 2,4 D,NAA, IAA.
出处
《华中师范大学学报（自然科学版）》
CAS
CSCD
北大核心
1997年第3期328-331,共4页
Journal of Central China Normal University：Natural Sciences
基金
国家自然科学基金
关键词
丹参
生长调节物质
脱分化
根芽分化
Salria miltiorrhiza Bunge
growth regulating substances
tissue culture
dedifferentiation
roots and buds differentiation
