摘要
采用琼脂块法、拌土育苗和蘸根盆栽生物试验及PPO活性测定法研究供试放线菌对病原菌的皿内拮抗作用、对草莓的促生作用及诱导抗性。结果表明：①供试放线菌对5种草莓土传病害病原菌有明显的抑制作用。Act11对5种病原菌均有较强的抑制作用;Act12对尖孢镰刀菌外的4种病原菌有拮抗作用,Act1对尖孢镰刀菌和草莓疫霉有拮抗作用。②生防菌剂接种促生作用明显,对根系生长的刺激效应尤为显著。在拌土接种育苗处理中,草莓根系及茎叶重量分别较对照提高122.4%~265.6%和53.6%~64.4%。③生防菌接种后可提高草莓新叶和根系PPO活性,产生诱导抗性;根系PPO活性增幅大于叶片。在拌土育苗和蘸根盆栽处理中,草莓新叶PPO活性较对照提高幅度均值分别为5.9%和16.9%;草莓粗根PPO活性提高幅度均值分别为21.4%和35.0%,细根PPO活性提高幅度均值分别为31.3%和26.3%。由此得出结论：供试生防菌在皿内能抑制草莓常见土传病病原菌生长;在拌土育苗和蘸根盆栽条件下均能显著促进草莓根系发育及茎叶生长,并使草莓产生诱导抗性。
Measure the inhibition by agar piece method. After puddling treatment raise seedlings and dipping root pot culture test, get the growth promoting effect to the strawberry and measure the strawberry leaves and root＇s PPO activity, so as to get the induced endurance. The results showed that： ①The three actinomyces strains all have transparent inhibition to the five pathogenetic fungi. Act11 has strong effect to the five disease germs. Act12 has inhibitory action to four disease except Fusarium oxysporun. Act1 has the same effect on both Fusarium and P. fragaride Hickm. ②The three actinomyces strains all have transparent growth promoting effect after the inoculation, especially to the root of the strawberry. The root weight rising by 122.4% to 265.6% over check test and the leaves-stem reach to 53.6% to 64.4%. ③The three actinomyces strains can promote the leaves and root＇s PPO activity, produce the induced endurance of the strawberry after the inoculation. The effect to the root＇s PPO activity is stronger than the leaves＇. After puddling treatment raise seedings and dipping root pot culture test, the three actinomyces strains can improve the strawberry new leaves＇ PPO activity in 5. 90% and 16.9% ,improve the strawberry thick root＇s PPO activity in 21.4% and 35.0%,as well as thin root＇s activity in 31.3% and 26.3%. The three actinomyces strains all have inhibition to the five strawberry pathogenetic fungi. After puddling treatment raise seedlings and dipping root pot culture test, they can improve each part＇s biomass of the strawberry plant and make the strawberry produce induced endurance.
出处
《西北农业学报》
CAS
CSCD
北大核心
2007年第6期146-153,共8页
Acta Agriculturae Boreali-occidentalis Sinica
基金
陕西省科技攻关项目(2004K02-G7)
关键词
草莓
生物防治
放线菌
拮抗
生物量
PPO活性
诱导抗性
Strawberry Biotic-control Actinomyces Suppression Biomass PPO activity Induced endurance
