摘要
2005年3—5月,在室内养殖条件下对第一次产卵后的中华绒螯蟹进行连续采样,系统研究了其第二次卵巢发育期间的卵巢指数(GSI)、肝胰腺指数(HSI)、卵巢外部特征的变化,并测定了卵巢和肝胰腺中的主要生化成分(水分、蛋白、脂肪和碳水化合物)的变化。结果表明:1.中华绒螯蟹第二次卵巢发育过程中,卵巢指数(GSI)显著增加(p<0.05),肝胰腺指数(HSI)先增加后减少,GSI与发育天数呈显著正相关性(p<0.01),HSI和GSI的变化呈显著负相关性(p<0.05)。2.第二次卵巢发育过程中,卵巢中的水分含量显著下降,脂肪、蛋白质和碳水化合物的含量显著上升(p<0.05);肝胰腺中的水分含量显著增加(p<0.05),脂肪和碳水化合物的含量显著下降(p<0.01),蛋白质含量基本不变。3.第二次发育过程中,卵巢中的脂肪、蛋白质和碳水化合物的含量与GSI呈正相关(p<0.01),卵巢中的水分含量与GSI呈显著负相关(p<0.01);肝胰腺中的脂肪和碳水化合物的含量随着HSI的下降而显著下降,肝胰腺中的水分含量与HSI呈显著负相关(p<0.01);肝胰腺中的脂肪含量与GSI及卵巢中脂肪含量均呈显著负相关(p<0.01),这说明中华绒螯蟹第二次卵巢发育过程中肝胰腺中的部分脂肪转运到二次卵巢中去。
Chinese mitten crab Eriocheir sinensis is a very important aquaculture species in China.Because of high edible yield,delicate flavour,excellent nutritional quality and rapid growth,the crab has been widely cultured and stocked in ponds,reservoirs and lakes after 1990's.As a consequence,the aquaculture production of this species had increased from 8,000 tons in 1991 to approximately 500,000 tons in 2004.Female E.sinensis is known to be able to spawn more than one batch of eggs after a single mating,which will take approximately one or two month to develop their ovaries for the second spawning after the first spawning.There are some differences between the first ovarian development and the second ovarian development of E.sinensis,i.e.the first ovarian development always lasts 3-4 months,while the second ovarian development only takes 1-2 months.Although there are many researches conducted to investigate the first ovarian development,only one available literature could be found on the second ovarian development of E.sinensis.So,the present study aimed to investigate the changes of the gonadosomatic index(GSI),hepatosomatic index(HSI),external features of ovary,main biochemical composition in ovaries and hepatopancreas during the second ovarian development of E.sinensis,which should provide valuable information for comprehending the reproductive biology and optimizing broodstock breeding on female E.sinensis.During March to May in 2005,the mature female E.sinensis were held in the indoor captive conditions and fed with fresh clam,Sinonovacula constricta.After the mating,the spawned females were sampled and dissected to investigate the changes of the GSI,HSI,external features of ovary.Furthermore,the main biochemical composition in ovaries and hepatopancreas were determined during the second ovarian development of E.sinensis.The results showed that:1.During the second ovarian development of E.sinensis,the GSI increased significantly from 0.82%in stageⅠto 5.42%in stageⅣ,which was significantly lower than the GSI in stageⅣ(GSI 10%—14%)during the first ovarian development.The ovary developed slowly during the berried period of female E.sinensis,which could be explained that the more energy was consumed by agitating their abdomens for embryonic development.2.However,the HSI rose slightly,then declined gradually during the period.The positive correlation could be found between GSI and days after spawning(DAS)and the negative correlation could be observed between GSI and HSI during the second ovarian development.3.The moisture in ovary decreased significantly,while the lipid,protein and carbohydrate content increased significantly.However the moisture in hepatopancreas increased significantly,the lipid and carbohydrate content decreased dramatically.4.The correlative analysis showed that there were significantly positive correlations between the lipid,protein,carbohydrate content and GSI,and the negative correlation was found between GSI and moisture in ovary.The similar trend was also found between those biochemical compositions in hepatopancreas and HSI except for protein level in hepatopancreas,which was constant(10%—12%of wet weight)during the second ovarian development.The lipid content in hepatopancreas and ovary was negative correlation during the period,which indicated that the hepatic lipid was transported to ovary during the second ovarian development of E.sinensis.
作者
于智勇
吴旭干
常国亮
成永旭
刘智俊
杨筱珍
YU Zhi-Yong;WU Xu-Gan;CHANG Guo-Liang;CHENG Yong-Xu;LIU Zhi-Jun;YANG Xiao-Zhen(Key Laboratory of Aquatic Genetic Resources and Aquaculture Ecosystem Certificated by the Ministry of Agriculture,Shanghai Fisheries University,Shanghai 200090,China)
出处
《水生生物学报》
CAS
CSCD
北大核心
2007年第6期799-806,共8页
Acta Hydrobiologica Sinica
基金
国家自然科学基金(399000112,30471349)
上海市教委重点学科项目(科04-130)
上海市重点学科项目(Y1101)联合资助
关键词
中华绒螯蟹
二次卵巢发育
卵巢指数
肝胰腺指数
生化成分
Eriocheir sinensis
The second ovarian development
Hepatosomatic index
Gonadsomatic index
Biochemical composition
