摘要
目的通过人类白细胞抗原（HLA）-B基因检测分析探讨广西地区范围哮喘儿童HLA-B等位基因遗传分布情况。方法将居住广西南宁地区无血缘关系的84例哮喘儿童和168例无哮喘和特应，性痰病的健康个体为对照组纳入研究。采用Pharmacia UniCAP系统检测哮喘儿童的血清总IgE水平，同时完成10种吸入性过敏原皮肤实验和肺功能检查。应用基因芯片法检测HLA-B的27个等位基因位点。计算疾病组和对照组的基因频率=基因出现频次数／（每组个体总数×2）×100％，进行χ^2检验和危险性分析。结果支气管哮喘组HLA-B56等位基因和HLA-B58等位基因频率（分别为5．36％和14．28％）明显高于健康对照组（分别为0．89％和8．04％），（χ^2值为7．973，P〈0．01和5．421，P〈0．05），比值比（OR）分别为6．6（95％可信限1．737～25．076）和2．09（95％可信限1．115～3．912）；而HLA-1346等位基因和HLA-B61等位基因频率在哮喘组（分别为6．55％和1．19％）低于对照组（分别为12．80％和4．76％），（χ^2值为5．197和4．308，P〈0．05），OR分别为0．438（95％可信限0．213～0．902）和0．232（95％可信限0．055～1．100）。结论HLA-B56等位基因和HLA-B58等位基因与广西南宁地区哮喘儿童易感性相关；HLA-B46等位基因对机体则可能有保护性。
Objective To investigate the characteristics of the genetic frequency distribution of human leukocyte antigen（HLA）-B alleles in Guangxi children with bronchial asthma. Methods Eighty-four blood-unrelated asthmatic individuals and 168 healthy controls without asthma and atopy living in Nanning region of Guangxi province were involved in the study. All the asthmatic patients had their serum total IgE levels measured with UniCAP Pharmacia system,and skin-prick test with ten kinds of inhalant allergens was taken, pulmonary function measured among the patients. HLA oligonucleotide array was used to 27 gene frequencies of HLA-B. Allelic frequency was calculated as follows：allelic frequency= n/（ N × 2） × 100%, （ n represents number of individuals carrying this allele, and N for total number of subjects in the groups）. Comparison of the distribution of HLA-B alleles between two groups was evaluated by Chi-square test, and the risk with asthma of the HLA alleles carriers was valuated by ORs value. Results The frequencies of HLA-B56 allele and HLA-B58 allele among the asthmatic patients were 5.36% and 14.28%, significantly higher than those in healthy controls （0.89% and 8.04%, χ^2=7. 973, P 〈0.01） and 5. 421, P 〈0.05）, odds ratios（ORs） were 6.6（95% CI：1. 737-25. 076） and 2.09（95% CI：1. 115-3. 912） respectively. HLA-B46 allele and HLA-B61 allele （6.55 % and 1.19 %） were significantly decreased in the asthmatics compared to those of healthy controls （12.80% and 4.76%,χ^2=5. 197 and 4. 308, P 〈0.05） ,ORs were 0. 438 （95% CI：0. 213-0. 902）and 0. 232 （95%CI：0. 055-1. 100）. Conclusion HLA-B56 allele and HLA-B58 allele are implicated in susceptibility to asthma in Nanning region of Guangxi province, HLA-B46 allele might be a protective factor against asthma.
出处
《临床荟萃》
CAS
北大核心
2007年第22期1624-1626,共3页
Clinical Focus
基金
广西壮族自治区自然科学基金项目资助(桂科自0339027)
国家自然科学基金项目资助(项目编号3026004/C011005)
