摘要
应用小鼠胚胎肢芽器官体外培养和计算机图像分析技术，通过体内／外和体外实验途径研究锌缺乏与过量的致畸性。结果表明：缺锌使多数肢体软骨原基发育受阻，软骨面积减小，外形发育受抑制，出现严重的缺指（趾）和并指（趾）。软骨和软组织均受影响。后肢比前肢受到的影响大；随着锌剂量的过度增加，肢体中各种软骨原基的发育分化越差、软骨面积越小，呈剂量反应关系。后肢比前肢易受影响，爪骨比长骨敏感，骨组织受到的影响比软组织严重。在体外实验中，锌含量在１０～１５ｍｇ／Ｌ（培养基）显示了致畸性。在体内／外实验中，５０ｍｇ／ｋｇｂｗ就开始对肢体产生影响。
Chelating agent (DTPA) or zinc sulfate(10,15,20mg/Zn L medium) was added to culture medium(in vitro studies),inducing either zinc deficiency or zinc excess in vitro respectively,limb buds from 12 day old mouse embryos(Kunming Strain) were cultured;pregnant mice were fed a zinc deficient diet(0.3-0.5μgZn/g diet) ad libitum(group ZD)or administered orally extra zinc sulfate at dose levels of 50,100,150mg Zn·kg bw -1 ·d -1 during day 7-11 of gestation(in vivo-and in vitro studies),limb buds of day 12 mouse embryos were cultured.After 3 days incubation,morphogenetic differentiation of the cultured limbs were examined and the area and forms of the bone anlagen were measured by image analyzer.The results showed that in the case of Zn deficiency,the growth of entire limb was inhibited,chondrogenesis delayed,the area of bone anlagen decreased,soft tissue was also affected,and the incidence of ectrodactylia and syndactylia was very high.All the above effects were found to be more severe in hindlimbs than in forelimbs.In the case of zinc excess,as Zn concentration increased the degree of differentiation and the area of both fore and hindlimbs were significantly decreased.The effect was also more severe in hindlimbs,paw bone was more susceptible than the long bones.Zinc excess showed greater effect on chondrogenic tissue than on soft tissue.In vitro,both 15mg Zn/L(in medium)and 50mg Zn/kg bw in vivo/vitro,can induce malformations.
出处
《营养学报》
CAS
CSCD
北大核心
1997年第2期196-201,共6页
Acta Nutrimenta Sinica
基金
国家教委博士点基金
关键词
锌缺乏
锌过量
器官培育
致畸性
Zn deficiency Zn excess organ culture teratogenicity image analysis
