摘要
目的：研究锌离子对破骨细胞体外吸收牙片功能的影响。方法：体外分离、培养新生乳兔破骨细胞，与玻片和灭活牙片共同培养，加入不同浓度锌离子。抗酒石酸酸性磷酸酶（TRAP）染色鉴定玻片上的破骨细胞，显微摄影分析破骨细胞吸收造成的牙片上的吸收陷窝，原子吸收分光光度法测定溶出的钙，并将实验组与对照组上清液钙离子浓度的比值定义为骨吸收指数，以评价破骨细胞的功能。结果：体外成功分离培养出多核的、TRAP（＋）的破骨细胞。破骨细胞吸收牙片时，首先在接近牙根牙骨质或牙本质部位开始形成吸收陷窝，这与这些部位的矿化程度相对较低有关；破骨细胞在牙片上形成的吸收陷窝与骨片相比，吸收陷窝数量较少，体积较小，多为正圆形；吸收深度较浅，常为大面积的浅吸收。用原子吸收分光光度法测定不同浓度的锌离子对溶出的钙和骨吸收指数的影响，初步结果表明，培养第3天，1×10^-4～1×10^-14mol／L锌离子刺激破骨细胞吸收牙片，其中1×10～mol／L，1×10^-10mol／L和1×10^-14mol／L锌离子能够显著刺激吸收（P〈0．05）；但是到了培养第7天，各浓度组除了对照组和1×10^-14mol／L锌离子还进一步有吸收外，其余浓度锌离子组的上清液钙离子浓度与自身第3天相比都有降低，但与同时期的对照组相比差异无统计学意义。在培养末期（第7天）1×10^-4～1×10^-7mol／L，1×10^-9mol／L，1×10^-12mol／L和1×10^-13mol／L浓度组的骨吸收指数小于1，而1×10^-8mol／L，1×10^-10mol／L，1×10^-14mol／L和1×10^-14mol／L浓度组骨吸收指数都大于1。结论：锌离子对破骨细胞吸收功能的作用与浓度和时程有关。
Objective：To investigate the bone resorption caused by osteoclasts and modulating functions of zinc ion on dental slices. Methods： Osteoclasts were separated from long-limb bones of neonatal rabbits, cultured with de-activated human tooth slices and glass slices. The cells in the experiment group were treated with 1×10^-14mol/L - 1 ×10^-4mol/L zinc ＋ 10% （volume fraction） fetal calf serum （FCS） ＋ αMEM, while those in the control group were grown in 10% FCS ＋ αMEM. Osteoclasts on glass slices were stained by TRAP staining. The absorption pits on tooth slices were observed by inverted phase contrast microscope. The resorbing activity was evaluated with the concentration of calcium in the supernatant liquid of osteoclasts. The ratio between the concentration of calcium in the experiment group and that of the control group was termed the resorption index. Results： The isolated cells were multinuclear and showed positive in cytoplasma by TRAP staining. Usually, osteoclasts resorbed tooth slices first on the cementum and dentin, which had lower content of mineralized tissue. Compared with those on bone slices, the lacunae on the dental slices appeared less in amount, less in area and shallower in depth. They often showed shallow pits in a large area. Microscopy showed that the number and area of absorption pits formed on treated tissues were less than those on the control tissues. The content of calcium in the supernatant liquid increased at the concentrations of 1×10^-4 -1×10^-14mol/L zinc, especially in the group of 1×10^-8mol/L, 1×10^-10mol/L,1×10^-14mol/L zinc on the 3rd day （P 〈0.05）. But they were reversed on the 7＇h day, except in the group of 1×10^-14mol/L zinc. At the end of culture, the resorption indexes of 1×10^-4- 1×10^-7mol/L,1 ×10^-9mol/L, 1× 10^-12mol/L and 1×10^-13mol/L group were lower than 1 ,but those of 1× 10^-8mol/L, 1 ×10^-10mol/L, 1 ×10^-11 mol/L and 1×10^-14mol/L group were higher than 1. Conclusion：The effect of zinc ion on osteoclastic resorption in dental slices is associated with phase and dosage elosely.
出处
《北京大学学报（医学版）》
CAS
CSCD
北大核心
2006年第6期644-647,共4页
Journal of Peking University:Health Sciences
基金
北京大学口腔医学院青年科研基金资助~~
关键词
破骨细胞
牙骨质
锌
骨密度
Osteoclasts
Dental cementum
Zinc
Bone density
