摘要
采用聚丙烯酰胺凝胶电泳法对３４头白唇鹿，５８头马鹿和１１头梅花鹿的血清淀粉酶同工酶进行了比较研究。结果发现：（１）３种鹿的血清淀粉酶都有ＡＭＹ１，ＡＭＹ２和ＡＭＹ３种同工酶，但白唇鹿ＡＭＹ１和ＡＭＹ２同工酶的相对迁移率小于马鹿和梅花鹿；（２）３种鹿的ＡＭＹ１同工酶都有ＡＭＹ１Ｆ和ＡＭＹ１Ｓ两种表型，以ＡＭＹ１Ｆ为优势表型（分别为６４．７１％，６８．９７％和８１．８２％）；（３）白唇鹿的ＡＭＹ２同工酶存在ＡＭＹ２ＡＡ，ＡＭＹ２ＡＢ和ＡＭＹ２ＢＢ３种表型，以ＡＭＹ２ＡＡ为优势表型（５０．００％）；（４）马鹿的ＡＭＹ２同工酶存在ＡＭＹ２ＡＡ，ＡＭＹ２ＡＢ，ＡＭＹ２ＢＢ和ＡＭＹ２Ｏ４种表型，梅花鹿存在ＡＭＹ２ＡＢ和ＡＭＹ２ＢＢ两种表型，以ＡＭＹ２ＡＢ为优势表型（分别为８９．６６％和９０．９１％）。
white lipped deers,58 red deers and 11 sikas were used to study the isozyme spectra of serum amylase by means of a polyacrylamide gel electrophoresis. The results were as follows:(1) There were three isozymes of serum amylase including AMY 1,AMY 2 and AMY 3 in the three species of deers and the relative move rate of AMY 1 and AMY 2 isozymes in the white lipped deer was less than that in the red deer and the sika. (2) The AMY 1 isozyme showed both AMY 1F and AMY 1S phenotypes in the three species of deers and the AMY 1F type was a preponderant phenotype(64.71%,68.97%,81.82% respectively). (3) The AMY 2 isozyme showed three phenotypes including AMY 2AA ,AMY 2AB and AMY 2BB in the white lipped deer and the AMY 2AA type was the preponderant phenotype(50.00%). (4) The AMY 2 isozyme showed four phenotypes including AMY 2AA ,AMY 2AB ,AMY 2BB and AMY 2O in the red deer and both AMY 2AB and AMY 2BB phenotypes in the sika,the AMY 2AB type was the preponderant phenotype(89.66% and 90.91% respectively).
出处
《中国畜牧杂志》
CAS
北大核心
1997年第5期15-17,共3页
Chinese Journal of Animal Science
基金
国家自然科学基金
关键词
鹿
血清
淀粉酶
同功酶
白唇鹿
马鹿
梅花鹿
Serum amylase Isozyme White lipped deer( Cervus albirostris ) Red deer (Cervus elaphus) Sika( Cervus nippon )
