摘要
采用光化学固定化方法将干扰素(IFN-γ)、肿瘤坏死因子(TNF-α)共固定化在12孔聚苯乙烯培养板(PSt)上.无血清培养子宫颈癌(HeLa)细胞,通过动态细胞计数跟踪、倒置显微镜观察、磷脂酰丝氨酸外翻分析方法,研究细胞因子药物对HeLa细胞凋亡诱导作用的长效活性.结果表明,20 ng/well的共固定化IFN-γ和TNF-α能显著诱导HeLa细胞凋亡,在18 d内,细胞发生了典型的凋亡,最高抑制活性可达94.12%,未见细胞有重新生长的现象.磷脂酰丝氨酸法分析也显示第3天和第6天的凋亡率为76.6%和88.7%.
The long - term inhibitory effect of co - immobilized IFN - γand TNF - α on the growth of HeLa cells is investigated. IFN - γ and TNF - α were co - immobilized on cell culture polystyrene and cells were incubated 18d on that. Cell counting was used to determine the inhibitory rate of co - immobilized cytokines on HeLa cells. Inverted microscope was used to observe the cellular form. The analysis for the exposure of phosphatidylserine was used to testify the apoptosis. The study showed that the co - immobilized IFN - γand TNF-α （20ng/well）could inhibit HeLa cells growth in a time -dependent manner, reached 94.12% inhibitory rate in 18 days and had significant activity of inducing HeLa cells apoptosis. The analysis for the exposure of PS showed that the apoptosis rates reached 76.6% and 88.7% after being cultured 3 and 6 days respectively.
出处
《华南师范大学学报（自然科学版）》
CAS
2007年第2期114-118,共5页
Journal of South China Normal University(Natural Science Edition)
基金
广东省科技资助项目(2006B35802003)
关键词
光固定化
人重组肿瘤坏死因子-α
重组人干扰素-γ
子宫颈癌细胞
磷脂酰丝氨酸外翻分析
长效性
photo - immobilization
tumor necrosis factor - α （ TNF - α）
interferon - γ （ IFN - γ）
cervical cancer cell line
the analysis for the exposure of PS
long - term effect
