摘要
直接用煮沸的细菌裂解液中的ＤＮＡ作ＰＣＲ模板，扩增效果与用酚／氯仿抽提纯化的质粒ＤＮＡ作模板的结果一致，缩短了实验时间。利用简单材料回收酶切片段，省去了有机溶剂反复抽提和沉淀的步骤，回收率达到９０％，效果很好。
The specific gene fragment as was amplified by PCR using overnight cultured bacteria boiling lysates as template directly. Amplification result of bacteria lysates was identical to that of extracted and purified plasmid DNA. This method curtailed the experimental time. Enzyme cleavage fragment was recovered from agarose gel with simple materials. This procedure omitted the repetition of extraction and precipitatioin by organic dissolvent. It was found that recovery rate was nearly 90% and the effect was sound.
