摘要
目的:观察染料木黄酮(GEN)对人卵巢癌裸鼠移植瘤内血管生成及肿瘤细胞的抑制作用。方法:建立20只裸鼠人卵巢癌移植模型,随机分成对照组、低剂量GEN组、中剂量GEN组和高剂量GEN组。GEN各组分别灌胃5mg(kg.d)-1(低剂量)、25mg(kg.d)-1(中剂量)和50mg(kg.d)-1(高剂量),共4周。对照组同体积去离子水灌胃。测定三组抑瘤率,用Ⅷ因子抗原多克隆抗体测定肿瘤内微血管密度(MVD),用细胞增殖指数(PI)反映细胞增殖状态,并通过电镜进行形态学观察。结果:GEN低剂量、中剂量和高剂量组移植瘤质量抑瘤率分别为1.90%、18.50%和38.07%。GEN中、高剂量组瘤重与对照组有明显差异(P<0.05)。GEN中、高剂量组的MVD和PI值与对照组比较,差异有显著性(P<0.05)。电镜观察可见GEN组随GEN剂量增加凋亡细胞、凋亡小体及变性坏死增多,而对照组少见。结论:GEN对人卵巢癌HO-8910PM细胞裸鼠移植瘤有明显抑制作用,并呈现剂量-效应关系;GEN对肿瘤细胞的作用,与抑制肿瘤细胞的增殖和肿瘤区新生血管的生长、阻断肿瘤血供有关。
Objective： To observe the inhibitory effects of genistein （GEN） on angiogenesis and growth of transplanted human ovarian cancer cells in nude mice. Methods： The transplantation models of human ovarian cancer in 20 nude mice were set up and were randomly divided into the control, lowdose GEN, medium-dose and high-dose groups. Intragastric administration was carried out in all the groups, with 5 mg/k·d in the low-dose group, 25 mg/k·d in the medium-dose group and 50 mg/k·d in the high-dose group. This treatment last 4 weeks. Intragastric administration of deionized water of the same volume was conducted in the controls. The inhibition rate in the 3 groups was observed. Polyclonal antibody of Ⅷ factor antigen was employed to detect the intratumoral macrovascular density （MVD）. Cell proliferation index（PI） was used to compare the state of cell proliferation. Histological features were observed with electron microscopy. Results： The inhibition rate in the low-dose, mediumdose and high-dose GEN groups was 1.9%, 18.50% and 38.07%, respectively. There was a significant difference in the weight of tumors between the control group and the medium-dose and high-dose GEN groups（P〈0.05）. There was a significant difference in MVD and PI values between the control group and the medium-dose and the high-dose GEN groups（P〈0.05）. Electron microscopy observation showed that with increased GEN dose, the number of apoptotic cells and denatured necrosis increased, which was seldom seen in the control group. Conclusion： GEN has an obvious inhibitory action on the human ovarian cancer cell line HO-8910PM transplanted in nude mice, with a dose-dependent effect. GEN inhibits tumor cell prolii＇eration and new vessel growth in the tumor area, thus blockading tumor blood supply.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2007年第21期1244-1247,共4页
Chinese Journal of Clinical Oncology
基金
国家自然科学基金资助(编号:30472226)
