摘要
目的:建立测定青蒿中青蒿素含量的紫外分光光度检测方法,并对不同产地的青蒿样品进行含量测定。方法:用紫外分光光度计对青蒿素标准溶液进行波长扫描,确定最适吸收波长并建立吸收度-浓度曲线。根据标准曲线测定青蒿样品中青蒿素的含量。结果:青蒿素的最适吸收波长为292 nm,吸收度-浓度回归方程为:A=0.05826C-0.00117,相关系数r=0.9999。结论:本法快捷、简便,可作为青蒿药材及青蒿素类产品的质量控制方法;不同栽培地青蒿药材中青蒿素的含量测定为青蒿药材的收购及栽培等提供依据。
Objective： To establish a simple method to determine artemisinin in Artemisia annua by ultraviolet spectrophotometrie and determine the conteat of artemisinin in Artemisia annua from different places by this new method. Methods： Carrying on the wave length scanning with the ultraviolet spectrophotometer to arteannuin standard solution, establishing the standard curve, according to standard curve determination sample content. Results ： The most suitable absorption wave length of artemisinin in Artemisia annua was 292 nm, and the extinction-density regression equation was： A = 0. 05826C -0. 00117,r = 0. 9999. Conclusion： The method of ultraviolet spectrophotometric determining artemisinin in Artemisia annua is rapid and convenient. It can be used for the quality control of this herbal medicine.
出处
《中药材》
CAS
CSCD
北大核心
2007年第10期1257-1259,共3页
Journal of Chinese Medicinal Materials
关键词
黄花蒿
青蒿素
紫外分光光度法
含量测定
Artemisia annua
Artemisinin
Ultraviolet spectrophotometric
Content determination
