摘要
目的：从紫荆花中分离、纯化总黄酮,对其黄酮成分及含量进行光谱分析。方法：以55%乙醇于100℃水浴回流提取紫荆花中的总黄酮,用聚酰胺树脂柱层析进行分离纯化,以芦丁、槲皮素、山柰酚为对照品,在200-600nm波长范围内进行紫外-可见吸收光谱扫描及含量测定,用KB r压片法在400-4000 cm^-1范围内进行红外光谱扫描。结果：粗黄酮平均得率为7.32%,纯化后的生药中总黄酮得率为2.20%;紫外-可见吸收及红外扫描光谱结果显示,紫荆花总黄酮纯品中含有芦丁、槲皮素、山柰酚3种黄酮甙元,含量分别为0.8271、0.2169、0.3007 mg/ml。结论：经聚酰胺树脂柱层析纯化的紫荆花黄酮纯品中的总黄酮甙含量为24.30%。总黄酮含量为0.5346%。
Objective： To separate, purify total flavonoids from Cercis chinensis Bge and analyze their flavonoid aglycones and content by spectrum method. Methods： The total flavonoids from Cercis chinensis Bge were extracted by 15 volume of 55% ethyl alcohol and hot water refluence at 100℃ and separated, purified by polyamide resin chromatography. Based on reference substances of rutin, quercetin, kaempferol, it was analyzed and detected by UV-Vis spectroscope at 200 -600nm and by KBr infrared spectroscope at 400 - 4000 cm^-1. Results ： Average extraction rate of crude total flavonoids was 7.32% , and extraction rate of purified total flavonoids was 2.20%. The results of UV-Vis and IR scanning spectrum showed that purified total flavonoids from Cercis chinensis Bge contained of 3 kinds of flavonoid aglycones of turin, quercetin, kaempferol, and their content was 0. 8271,0. 2169,0. 3007mg/ml respectively. Conclusion： Content of total flavonoid aglycones in purified flavonoids of Cercis chinensis Bge was 24. 30% , and content of total flavonoid was 0. 5346%.
出处
《中药材》
CAS
CSCD
北大核心
2007年第10期1252-1255,共4页
Journal of Chinese Medicinal Materials
基金
广东省湛江市科技攻关项目(No2005042)
关键词
总黄酮
紫荆花
纯化
黄酮成分
光谱分析
Total flavonoid
Cercis chinensis Bge
Purify
Flavonoid
Spectrum analyze
