摘要
目的探讨黄芩中黄芩苷的最佳提取工艺。方法选取温浸法、煎煮法、加碱温浸法中较佳方法,考察粒度、次数、提取时间和次数、分离方式等因素对黄芩苷收率的影响。结果煎煮法效果优于其他两种方法,煎煮法提取黄芩苷的最佳工艺条件为:粒度40目,煎煮2次,物料比1∶10和1∶5,时间60min和30min,双层滤布过滤再离心分离,70℃保温60min,酸沉静置3h抽滤,50%乙醇洗涤2次,干燥得黄芩苷产品。黄芩苷收率可达13.67%,其含量为85.7%,黄芩苷在pH3.98低温水浴液中性质较稳定。结论该实验结果为大规模提取生产黄芩苷提供理论依据。
Objective To investigate the best extracting technology of baicalin from Scutellaria baicalensis Georgi. Methods The best extraction method was selected from controlled temperature water immersing,decoction and controlled temperature alkali added water immersing method, and the affecting factors on yield and content of baicalin were investigated. Results The decoction method was better than others, the good technology conditions were as follows ： decocted the Scutellaria baicalensis 2 times with decuple water in 60 min and 5 folds water 30 min,respectively, then removed the residue with double filtration cloth and centrifuged, kept the temperature at 70℃ for 60 min,laid aside 3 hours, separated with centrifuge,dried the residue and obtained the baicalin product. The yield and content of baicalin was 13.67% and 85.7% respectively,the baicalin was stable at pH 3.98. Conclusion The results provide a theoretical basis for large - scale prodution of baicalin.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2007年第11期2664-2666,共3页
Lishizhen Medicine and Materia Medica Research
基金
中国西南地区资源植物的开发与利用国际合作资助项目(No2004DFB00300)
关键词
黄芩
黄芩苷
提取工艺
稳定性
Scutellaria baicalensis Georigi
Baicalin
Extracting technology
Stability
