摘要
目的观察双氢青蒿素(DHA)对阴道毛滴虫超微结构的影响。方法在2.5×106个/ml滴虫培养液中加入双氢青蒿素1 mg/ml。37℃培养3和3.5 h,进行扫描电镜观察,培养2.5和4 h进行透射电镜观察。结果双氢青蒿素作用滴虫后,虫体细胞膜受损明显,表面皱褶加深、表膜凹陷,并出现裂隙。细胞核膜破损,细胞核和细胞质均出现裂隙。内质网呈囊状肿胀,氢化酶体受损、变形。细胞质从细胞膜破裂处溢出。细胞膜剥脱后细胞核、氢化酶体和盾等结构裸露。最后虫体变性、坏死。结论双氢青蒿素能破坏阴道毛滴虫膜系结构并导致虫体裂解死亡。
Objective To observe the effect of dihydroartemisinin （DHA） on the ultrastrncture of Trichomonas vaginalis cultured in vitro. Methods The trophozoites of T.vaginalis were cultivated with liver extract solution medium containing 1 mg/ml dihydroartemisinin, and were then observed by scanning and transmission electron microscopes after the treatment for 2.5-4.0 h. Results The cell membranes of the trophozoites treated with DHA were damaged considerably. The surface of T. vaginalis showed deepening folds, hollow, and cracks. The nuclear membrane appeared fractures. There were a few of crevices in the nucleus and cytoplasm. Disordered and dilated endoplasmic reticulum, injured and deformed hydrogenosomes were found. Cytoplasm of the damaged parasites spilled over from torn place. After the cell membrane was peeled off, the nuclei, hydrogenosomes and peha were exposed, which finally resulted in the death of the denatured parasites. Conclusion Dihydroartemisinin can destroy membrane structure and organelles of Trichornonas vaginalis trophozoites, which leads to decomposition and necrosis of the parasites.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
2007年第1期41-44,共4页
Chinese Journal of Parasitology and Parasitic Diseases
基金
江西省教育厅科技项目[2004]69~~
关键词
阴道毛滴虫
双氢青蒿素
超微结构
扫描电镜
透射电镜
Trichomonas vaginalis
Dihydroartemisinin
Ultrastructure
Scanning electron microscope
Transmission electron microscope
