摘要
建立了高效毛细管电泳检测盐酸安妥沙星中对映体杂质的方法。以含20mmol／LHP-β-CD与70mmol／L磷酸二氢钾溶液（用磷酸调节pH3．0）为运行缓冲液，电压30kV，柱温30℃，检测波长297nm。可在10min内将两对映体良好分离，检出限为2mg·L^-1（S／N=3），左旋安妥沙星在4．89—61．2mg·L^-1呈良好的线性关系，日内精密度（RSD）为2．1％（n=5），日间精密度（RSD）为3．2％（n=15）。
Antofloxacin hydrochloride is a new chiral drug. A sensitive, simple and accurate method for enantiomeric impurity determination of antofloxacin hydrochloride was firstly developed by capillary electrophoresis. The optimum experimental conditions were 20 mmol/L HP-β-CD, 70 mmol/L phosphate buffers （ pH 3.0） with an effective voltage of 30 kV at 30 ℃ and detected at 297 nm. The method offers good separation of the two enantiomers in less than 10 min. The limit of detection was 2mg·L^-1（S/N=3）. Good linear ranges were obtained from 4.89 to61.2mg·L^-1. The relative standard deviations of intra- and inter-day were 2. 1% （n =5） and 3.2% （n = 15）.
出处
《分析测试学报》
CAS
CSCD
北大核心
2007年第6期888-890,共3页
Journal of Instrumental Analysis
关键词
盐酸安妥沙星
对映体杂质
高效毛细管电泳法
： antofloxacin hydrochloride
enantiomeric impurity
capillary electrophoresis
