摘要
蜂毒多肽在肿瘤治疗中的应用引起研究者的极大兴趣。本研究使用大豆磷脂、胆固醇、羧酸化PEG-胆固醇制备了蜂毒多肽空间稳定脂质体,并将二硫键稳定抗人肝癌单链抗体联结PEG-胆固醇末端。使用酶联免疫法考察了蜂毒多肽空间稳定免疫脂质体的活性。蜂毒多肽空间稳定免疫脂质体有较高的肿瘤细胞选择性。体外实验证明,其对SMMC-7721细胞的杀伤能力远强于蜂毒多肽空间脂质体,而对Hela细胞的杀伤能力与蜂毒多肽空间脂质体无区别。蜂毒多肽空间稳定免疫脂质体对肿瘤细胞的选择性,可使其成为一种有效的靶向制剂。
Recently the use of peptides in bee venom （PBV） for cancer therapy has attracted considerable attention. In this study, the sterically stabilized liposomal PBV （PBV-SL） was prepared using soybean phosphatidylcholine, cholesterol, and cholesterol-PEG-COOH. The humanized anti- hepatoma disulfide-stabilized Fv （hdscFv25） was coupled to sterically stabilized liposomes using the N-hydroxysuccinimide ester method. The hdscFv25-immunoliposomes （ SIL[ hdscFv25 ]） were immunoreactive as determined by ELISA assay. SIL[ hdscFv25 ] showed higher tumor cells selectivity. PBV-SIL[ hdscFv25 ] can kill SMMC-7721 cells in vitro with higher efficiency than non-targeted liposomes. Whereas cytotoxicties were compared for Hela cells, no significant differences was observed between PBV-SIL[ hdscFv25 ] and PBV-SL. Sterically stabilized immunoliposomal peptides in bee venom could be one drug targeting delivery system.
出处
《药学学报》
CAS
CSCD
北大核心
2007年第11期1201-1205,共5页
Acta Pharmaceutica Sinica
关键词
蜂毒多肽
空间稳定免疫脂质体
肿瘤细胞选择性
体外细胞毒作用
peptide in bee venom
sterically stabilized immunoliposome
tumor cells selectivity
in vitro cytotoxicity
