摘要
枣疯病是中国枣区最重要的病害之一,从表现症状的和未表现症状的3个枣品种(酸枣、辣椒枣和郎枣)的叶脉中提取核酸DNA。用13个引物(12个10 bp碱基和1个11 bp碱基)进行RAPD分析。只有引物AM-14从所收集的表现症状枣品种中扩增出400 bp的特异条带,而其他12个引物未有基因多样性;引物AL-07可以区分郎枣和其他2个品种,所有的样品重复2次,结果一致,与使用植原体特异性引物的常规PCR相比,对于检测不同枣品种枣疯病的枣植原体,RAPD技术是一个快速有效的方法。
Jujube witches＇ broom is a devastating disease of Ziziphus jujube that occurs in various jujube regions of China. Nucleic acid extracted from midribs of samples collected from three jujube varieties（Suanzao, Lajiaozao, Langzao） from symptomatic and asymptomatic shoots were tested by random amplified polymorphic DNA analyses. Using 13 different 10 and 11 bp random primers the amplification of jujube DNA was achieved from all the samples;AM-14 primer provided amplification of specific DNA fragments of about 400 bp, only from samples collected from symptomatic plants. No genetic variations in these varieties were identified using the other 11 arbitrary primers ; only with primer AL-07 it was possible to differentiate Lang-zao from the other two varieties tested. All the experiments were repeated 2 times and the results were consistent. Compared with PCR analyses with phytoplasma-specific primers, RAPD techniques resulted to be an alternative rapid and sensitively method for detecting jujube phytoplasmas presence in different jujube varieties.
出处
《华北农学报》
CSCD
北大核心
2007年第5期172-175,共4页
Acta Agriculturae Boreali-Sinica
基金
山西省国际科技合作项目(2006081004)
关键词
枣疯病
植原体
RAPD
检测
Jujube witches＇ broom
Phytoplasmas
Random Amplified Polymorphic DNA
Detection
