摘要
在以基因枪转化获得的抗除草剂转bar基因材料HR为供体亲本;以9311为受体亲本的回交转育过程中,发现回交转育后代9311HR的抗性单株自交后代中bar基因始终表现1∶1的异常分离。以外源基因异常分离水稻9311HR为材料,探讨bar基因的导入对其主要农艺性状、花粉育性、花粉萌发率的影响。结果表明:抗除草剂bar基因的导入对目标亲本的主要农艺性状无明显影响,9311HR抗性植株正常染色花粉率及花粉离体萌发率均在90%左右,其结实率正常,与轮回亲本9311均无显著差异。花粉DNA的PCR分析结果显示,9311HR抗性植株形成了携带外源基因bar的成熟花粉。说明9311HR抗性植株不存在雌雄配子败育。
Abnormal segregation of foreign gene bar was observed in a herbicide resistant rice 9311HR developed by transferring the bar gene from the donor, a genetically modified organism （GMO） HR obtained by biolistic method, into the breeding line 9311 by backcrossing. The effects of foreign gene bar on main agronomic traits, pollen fertility and pollen germination were studied in abnormal segregating rice 9311 HR. The foreign gene bar had no significant effect on the main agronomic traits of herbicide-resistant plants of 9311 HR. The pollen fertility and in vitro pollen germination rate for herbicideresistant plants of 9311HR were about 90%. Also, the herbicide-resistant plants of 9311HR showed normal seed fertility. There was no significant difference in pollen fertility, in vitro pollen germination rate and seed fertility between 9311HR and 9311. Moreover, PCR analysis of pollen DNA indicated that mature pollen carried with foreign gene bar in herbicide-resistant plants of 9311 HR. Above results suggested that there was no gametal sterility in herbicide-resistant plants of 9311 HR.
出处
《江苏农业学报》
CSCD
北大核心
2007年第5期381-384,共4页
Jiangsu Journal of Agricultural Sciences
基金
国家"863"项目(2003AA207020)
江苏省高技术研究计划(BG2001304
BG2004304
BG2005301)
江苏省植物功能基因组学重点实验室开放课题(02738800338)
关键词
水稻
BAR基因
异常分离
花粉育性
rice （ Oryza sativa L. ）
bar gene
abnormal segregation
pollen fertility
