摘要
目的:观察复合营养素配方对老龄大鼠认知与运动功能的改善效果,并初步阐明其作用机制。方法:24只15月龄Wistar大鼠,随机分为干预组和对照组,每组雄性、雌性各6只。干预组大鼠饲喂添加复合营养素的自制饲料,同时灌胃植物提取物混合液;而对照组饲喂基础饲料,同时灌胃蒸馏水。灌胃剂量为4ml/(kgbw·d)。实验期10w。采用水迷宫、rodwalking方法检测大鼠的认知及运动行为;碱性羟胺比色法检测脑组织乙酰胆碱(ACh)含量;荧光分光光度法检测脑组织去甲肾上腺素(NE)、5-羟色胺(5-HT)、多巴胺(DA)含量;血清及脑组织乙酰胆碱酯酶(ACHE)活性按试剂盒说明测定;神经电生理法观察海马齿状回长时程增强效应(LTP)的改变。结果:与对照组比较,干预组老龄大鼠的认知与运动功能得到改善;脑组织ACh、NE、5-HT、DA含量升高,血清及脑组织中AChE活性显著下降;大鼠海马齿状回LTP幅度明显提高,海马突触传递效能增强。结论:复合营养素对老龄大鼠的认知与运动功能有改善作用,其作用途径与增加神经递质含量,改善神经突触传递效率有关。
Objective： To investigate the improved effects of combined nutrients in aged rats with impaired cognition and motor function and illustrate its possible mechanism. Method： Twenty four Wistar rats were randomly divided into intervened group（15 mo old） and control group（3 mo old）, six male and six female in each. The rats of intervened group were fed with diets supplemented with combined nutrients while the control group with basic diet. 4ml/（kg ·bw ·d） mixed plants extract and water were administered intragastically to intervened and control group respectively. The experiment lasted 10 w. The cognition and motor behavior were tested with water maze and rod walking test. The level of acetylcholine in brain was determined by alkaline hydroxylamine colorimetry and noradrenaline, serotonin and dopamine by fluorospectrophotometry. The activity of acetylcholine esterase in serum and brain was tested according to the instruction of kits. The changes of long-term potentiation of hippocampal dentate gyrus were observed through electrophysiology. Results： Compared with control group, the cognition and motor function of aged rats in intervened group were improved. The contents of acetylcholine, noradrenaline, serotonin and dopamine in brain were increased while acetylcholine esterase activity lowered in serum and brain. The amplitude of LTP of hippocampal dentate gyrus and the efficacy of hippocampal synaptic transmission were significantly elevated. Conclusion： The combined nutrients have improved effects on cognition and motor function in aged rats, and the mechanism may be correlated with elevated neurotransmitter level and efficacy of synaptic transmission.
出处
《营养学报》
CAS
CSCD
北大核心
2007年第5期422-425,共4页
Acta Nutrimenta Sinica
基金
2004年度中国营养学会基金
关键词
认知
复合营养素
神经递质
长时程增强
cognition
combined nutrients
neurotransmitter
long-term potentiation
