摘要
目的探讨雷公藤内酯醇(TP)抗炎及免疫抑制作用的机制。方法以 Criess 试剂检测 TP 对小鼠腹腔激活巨噬细胞在不同剂量和不同时间段一氧化氮(NO)分泌的影响。结果在1～10μg/ml 范围内,TP 对腹腔激活巨噬细胞 NO 产生具有明显抑制作用(P<0.01),呈剂量和时间依赖性,且无细胞毒性。结论 TP 能有效抑制小鼠腹腔液中激活巨噬细胞 NO 生成,提示 TP 的抗炎及免疫抑制作用与抑制 NO 的生成有关。
Objective To explore the mechanism of the anti-inflammation and immune inhibition effect of Triptolide(TP).The effect of TP with different dosages and at different time points on NO secreted by activated peritoneal macrophages. MethodNO content in the supemate was determined by Griess reagent.Results TP had obvious inhibitory action on the production of PEM ΦNO with the range of 1-10/μg/ml(P<0.01),showing a dosage and time dependence without any cytotoxicity.Conclusion TP could effectively inhibit the pr...
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2004年第S1期164-166,共3页
Chinese Journal of Integrated Traditional and Western Medicine
