摘要
目的探讨康脑灵胶囊对痴呆大鼠神经元自由基损伤的保护作用及对caspase-3、Bcl-2表达的影响。方法建立血管性痴呆大鼠模型,中药干预后,用分光光度计检测超氧化物歧化酶(SOD)、丙二醛(MDA)、一氧化氮(NO)的含量,荧光分光光度计检测caspase-3表达,流式细胞技术检测Bcl-2的表达。结果康脑灵胶囊明显减少痴呆大鼠MDA含量,提高SOD、NO含量,降低caspase-3表达,增强Bcl-2表达。结论康脑灵胶囊对缺血再灌注痴呆大鼠自由基损伤有明显保护作用,其作用机制与增强Bcl-2表达、降低caspase-3表达有关。
Objective To study the protection of Kangnaoling capsule to free radical injury and influence on the expression of caspase-3 and Bcl-2 in neuron of VD rat. Methods VD rat model was duplicated. Using spectrophotometry to detect contents of SOD, MDA and NO, spectro-fiuorimeter to detect the expression of caspase-3, spectrophotometric method to detect the expression of Bcl-2 after traditional Chinese medicine interference. Results Kangnaoling capsule can markedly reduce the contents of MDA, raise contents of SOD and NO, degrade the expression of caspase-3, enhance the expression of Bcl-2. Conclusions Kangnaoling capsule can markedly protect VD rat from free radical injury, which probably correlated with increasing expression of Bcl-2, decreasing expression of caspase-3.
出处
《中国中医药信息杂志》
CAS
CSCD
2007年第11期21-22,共2页
Chinese Journal of Information on Traditional Chinese Medicine
基金
山东省教育厅课题(J01K06)
