摘要
目的:应用流式细胞仪从孕妇外周血中分离胎儿有核红细胞(NRBCs),通过荧光原位杂交(FISH)分析进行无创性产前诊断。方法:对15例妊娠11～24周的高危孕妇外周血按常规密度梯度方法分离单个核细胞,选用红细胞特异性抗体CD71、HbF结合流式细胞术(FACS)分选富集NRBCs,并进行FISH分析。结果:所有标本均分选出NRBCs,分选率为100%,CD71和HbF双阳性细胞含量为(3.43±1.62)×10-2。经FISH成功分析13例,未发现异常结果。结论:从孕妇外周血中分离富集得到的胎儿有核红细胞,通过FISH分析检测胎儿特定的染色体,可进行无创性产前诊断遗传性疾病。
Objective： To separate fetal nucleated red blood cells from the peripheral blood of pregnant women with FACS and FISH is feasible for noninvasive prenatal diagnosis of genetic diseases. Methods： Mononuclear cells in peripheral blood samples from 15 pregnant women from 11 to 24 weeks of gestation we：e isolated useing density gradient centrifugation. NRBCs were sorted with fluorescene - activated cell sorting （FACS） by erythrocyte specific antibody CD71 and fetal specific antibody HbF. Fluorescenece in situ hybridization （FISH） was performed. Results： Fetal NRBCs were separated from all cases and the content of CD71 and HbF double -positive cells was （3. 43 ± 1. 62） ×10^ -2. 13 cases were performed by FISH and abnormality was not detected. Conclusion： Te method that isolating and enriching fetal NRBCs from the peripheral blood of pregnant women and detecting special chromosomes of foetus by FISH is used to diagnose genetic diseases.
出处
《中国妇幼保健》
CAS
北大核心
2007年第31期4458-4459,共2页
Maternal and Child Health Care of China
基金
深圳市科技局立项课题编号:200204074
