摘要
用琼脂糖白细胞趋化实验法研究不同浓度的氧氟沙星、丹参酮Ⅱa及氢化可的松体外对人白细胞游走的影响。结果显示，3种药物体外对人白细胞的趋化游走均有明显的抑制作用，其抑制作用呈明显的剂量依赖性，并具有很好的直线相关性。其IC50丹参酮Ⅱa为0．46μg／ml，氧氟沙星为0．63μg／ml，氢化可的松为46.52μg／ml，表明体外抑制作用丹参酮Ⅱa最强，氢化可的松最弱。
In order to verify the possible anti-inflammatory activity and their potency of ofloxacin and Tanshinone Ⅱ a, the effects of different concentrations of ofloxacin, Tanshinone Ⅱ a and hydrocortisone on human leukocyte migration in vitro were investigated employing agarose migration technique in which f-Met-Lea-Phe was served as chemotactic factor. The results showed that all the three agents could inhibit migration of leukocytes remarkably. The ef-fects were concentration-dependent with excellent linear relationship. The IC50 were: Tan-shinone Ⅱa 0. 46 μg/ml, ofloxacin 0. 63 μg/ml, and hydrocortisone 46. 52 μg/ml respective-ly. The results indicated that Tanshinone Ⅱ a was the most potent agent for inhibiting migra-tion of human leukocytes in vitro and the hydrocortisone was relatively less potent.
出处
《中国医学科学院学报》
CAS
CSCD
北大核心
1997年第3期232-235,共4页
Acta Academiae Medicinae Sinicae
