摘要
目的以检测toxR基因作为副溶血性弧菌种水平上的特异性基因鉴定,建立一种快速、准确、简便的副溶血性弧菌(VP)筛选方法。方法根据toxR基因序列设计合成引物,扩增被检VP所含的相应基因。分别用标准株及地方株做对照,同时与常规鉴定方法比较。结果对浙江省2005年收集的286株来自临床病人和海产品的可疑VP鉴定,用常规方法鉴定出VP占总数的62.24%;用toxR基因检测,鉴定率占所有菌株的61.54%;两者差异无统计学意义(χ2=0.03,P>0.05),对40份海产品增菌液直接检测toxR基因与常规增菌分离鉴定法,均有37份标本检出VP,检出率为92.5%。结论该检测方法快捷、特异性好、敏感性高,可以作为VP快速筛选方法。
Objective This study was conducted m establish a rapid, simple and accurate method for screening Vibrio parahaemolyticus （VP） by the detection of the specific toxR gene of VP at a species level. Methods Corresponding genes carded by the tested VPs were amplified with primers synthesized based on the sequence of toxR gene. Standard strain and local strain were used as controls, and routine method was used for comparison. Results A total of 286 suspicious VP strains isolated from clinical patients and seafood in Zhejiang Province in 2005 were subjected to VP identification. A total of 62.24% of the strains were identified as VP using the routine method, while the positive rate was 61.54% by using toxR gene detection; there was no statistical difference between them （χ^2=0.03, P〉0.05）. When both of the routine isolation method and the toxR gene detection were performed respectively on 40 samples of bacterial enrichment liquid from seafood, 37 samples were tested VP positive with both of the methods, and the positive rate was 92.5%. Conclusion The toxR gene detection proved to be a simple, specific and sensitive method for the rapid VP screening.
出处
《疾病监测》
CAS
2007年第9期642-645,共4页
Disease Surveillance
关键词
副溶血性弧菌
toxR基因
分离培养
PCR
Vibrio parahaemolyticus
toxR gene
isolation and culture
polymerase chain reaction
