摘要
以盆栽2年生夏腊梅实生苗为材料,分对照、轻度胁迫、中度胁迫、重度胁迫和深度胁迫5组,研究土壤干旱对夏腊梅叶片脂质过氧化及保护酶活性的影响。结果表明:中度干旱胁迫下夏腊梅抗氧化能力增强,膜脂过氧化程度几乎不变;重度干旱胁迫下,脯氨酸含量、SOD和POD活性升高,还原型谷胱甘肽、抗坏血酸、类胡萝卜素含量和CAT活性下降,超氧阴离子产生速率和H2O2含量升高,导致丙二醛含量升高,膜透性增大,净光合速率、最大光化学效率、表观量子效率降低。重度干旱胁迫引起膜脂严重过氧化,对夏腊梅造成一定伤害。
Calycanthus chinensis is an endangered species which has survived from the tertiary period, and now it is only distributed in Zhejiang Province of China. In order to provide a scientific basis for conservation and cultivation of the plant, the effects of soil drought stress on antioxidant system and lipid peroxidation in leaves were investigated using 2-year-old pot-cultured seedlings. Fifteen pots of the seedlings with a similar growth rate were subjected to different levels of drought stress （ no stress, mild stress, moderate stress, severe stress and extremely severe stress）. In comparison with no stress and mild stress, antioxidant ability in leaves of C. chinensis significantly increased, while lipid peroxidation remained steadily under moderate stress. Proline content, superoxide dismutase （SOD） and peroxidase （POD） activities significantly increased, while the content of glutathione （GSH）, ascorbic acid and carotenoid and catalase （CAT） activity significantly decreased under severe stress. As a result of increase in generating rate of superoxide anion （O2^-） and content of hydrogen peroxide （H2O2 ）, membrane permeability of leaf cells and malondialdehyde （MDA） content increased, while net photosynthetic rate （P.）, maximal photochemistry efficiency （ Fv/Fm ） and apparent quantum yield （AQY） decreased to different extents. The present conclusion is that C. chinensis growth could be inhibited by enhancing lipid peroxidation under severe and extremely severe drought stress.
出处
《林业科学》
EI
CAS
CSCD
北大核心
2007年第10期28-33,共6页
Scientia Silvae Sinicae
基金
浙江省自然科学基金资助项目(Y504220)
关键词
夏腊梅
干旱胁迫
脂质过氧化
保护酶
Calycanthus chinensis
drought stress
lipid peroxidation
defense enzyme
