摘要
建立了一种快速简便的测定土壤中铵态氮的方法。土壤中的有效氮加碱蒸馏,用硫酸吸收,全部转化为铵态氮,NH4+在碱性溶液中能还原ClO-,该反应和化学发光反应ClO--Luminol相耦合,通过测定剩余的ClO-来达到间接测定NH4+的目的。该方法的检测下限达到10-3mg/kg,线性范围0.01～1.5mg/kg,相关系数为-0.9995,加标回收率在85.8%～94.5%之间,RSD<2.7%(n=5),完全能满足测定土壤中有效氮的需要。
A luminol-ClO^- chemiluminescence （CL）method for the determination of available nitrogen in soil with high sensitivity was developed. It was found that NH4^＋ could reduce ClO^- in the base solution. A new sensitive and selective CL method for the determination NH4^＋ in the dry soil was found based on the reductive reaction. The linear-response range of the method was 0.01-1.50mg/kg with a linear correlation coefficient of - 0.9989.The detection limit was 1 × 10^-3mg/kg. The RSD was less than 2.7%（n=5）. The recovery of the method was 85.8%～94.5%. The method was applied to the detection of available nitrogen in soil samples with satisfactory results.
出处
《中国农学通报》
CSCD
2007年第11期228-231,共4页
Chinese Agricultural Science Bulletin
基金
科技部创新基金"重金属
亚硝酸盐
农药残留综合快速测定"(07C26214100634)
关键词
化学发光
土壤
有效氮
chemiluminescence
soil
available nitrogen
