摘要
目的:研究调肝方药白香丹对体外培养的胚胎大鼠皮层神经元细胞活力及其5羟色胺1A受体(5-HT1AR)蛋白表达水平的影响。方法:制备白香丹大鼠含药血清,体外原代培养胎鼠大脑皮层神经元细胞,MTT法测定神经元细胞活力;免疫荧光标记+激光共聚焦检测法测定神经元中5-HT1AR蛋白水平表达量及定位分布。结果:白香丹含药血清能够显著提高体外培养的胚胎大鼠皮层神经元细胞活力,上调其5-HT1AR蛋白表达水平。结论:大鼠脑皮层神经元中5-HT1AR是调肝方药白香丹的作用靶位之一,白香丹可能通过提高神经元细胞活力并上调其5-HT1AR蛋白表达水平发挥疗效。
Objective:To study the effect of Baixingdan on expression level of Serotonin 1A receptor(5-HT1AR) and corpuscular viability of embryo rat cerebral cortex neural cells cultured in vitro.Methods: Prepare Baixiangdan medicated serum,culture embryo rat cerebral cortex neuron and assay the viability of neural cells by MTT;the expression levels of 5-HT1AR in the neural cells were evaluated by immunofluorescence and Laser Scanning Confocal Microscope techniques.Results: Baixiangdan medicated serum could markedly increase the viability of rat cerebral cortex neural cells,up-regulate the expression of 5-HT1AR in neuron.Conclusion: There is at least one important component targeting 5-HT1AR in Baixiangdan,and Baixiangdan might partially play its role in ameliorating premenstrual syndrome by increase the viability of rat cerebral cortex neural cells and up-regulating the expression of 5-HT1AR in them.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2007年第5期151-153,共3页
Pharmacology and Clinics of Chinese Materia Medica
基金
国家重点基础研究发展规划"973计划"项目(No.2006CB504804)
