摘要
目的:以磷酸钙骨水泥为原料制备一种生物型纳米根管封闭剂,并对其理化性质、显影效果及对感染根管优势菌的体外抑菌能力进行研究。方法:根据ISO 6876:2001(E)口腔生物材料标准,测定其流动性、凝固时间和晶粒大小,测定不同显影剂比例的材料的显影效果。采用NCCLs推荐的厌氧菌药物敏感试验,用琼脂稀释法研究其对内氏放线菌、厌氧消化链球菌、牙龈卟啉单胞菌、牙髓卟啉单胞菌和具核梭杆菌的体外抑菌效果。应用染色渗漏法测定20颗离体牙的封闭效果,并与氧化锌丁香油糊剂进行比较。采用SPSS12.0软件包对数据进行t检验。结果:调和后的新型纳米根管封闭剂固化后的终产物为羟基磷灰石,晶粒大小为279nm,具有较好的流动性,可操作时间大于30min,凝固时间为(1.0±0.5)h(可调控)。其显影效果良好。MIC≤0.156～0.312。试验组染色渗漏深度显著小于氧化锌丁香油糊剂组(P<0.01)。结论:制备的材料符合新型纳米根管封闭剂的要求。
PURPOSE： The purpose of this study is to develop a novel root canal filling sealers based on calcium phosphate cement （CPC）, and to evaluate its physical-chemical properties and in vitro antibacterial activity on the predominant bacteria infecting root canal. METHODS： The fluidity and the setting time of the sealer were tested according to ISO 6876：2001 （E） standards. The crystal size of the final product was determined. Its opacification with different composition were measured. The in vitro antibacterial property of the sealer was tested according to the Antimicrobial Susceptibility Testing of Anaerobes recommended by National Committee for Clinical Laboratory Standards （NCCLs）.The involved bacteria included Actinomyces naeslundii（A, naeslundii）,Peptostreptococcus anaerobius（P anaerobius）, Porphyromonas gingivalis （P. gingivalis）, Porphyromonas endodpntalis （P. endodpntalis） and Fusobacterium nucleatum （F nucleatum）.Twenty single-rooted human extracted teeth were selected to evaluate the sealing ability using dye microleakage technology. Dye penetration was measured and the results were statistically analyzed using SPSS12.0 software package. RESULTS： The new root canal filling sealer was primarily composed of hydroxyapatite in 279nm after setting. Its liquidity was suitable, the operating time was over 30 minutes, and the controlled setting time was （1.0±0.5） hours. The opacification was acceptable. MIC ≤0.156-0.312. The dye penetration was significant different between two filling types （P〈0.01）. CONCLUSIONS： The new root canal filling sealer is an ideal root canal filling sealer.Supported by Science and Technology Development Fund from the Science and Technology Bureau of Xiamen City（Grant No.2002-60-5）.
出处
《上海口腔医学》
CAS
CSCD
2007年第5期530-533,共4页
Shanghai Journal of Stomatology
基金
厦门科技局资助厦门科技发展课题(2002-60-5)
关键词
根管封闭剂
磷酸钙骨水泥
流动性
凝固时间
抗菌性
微渗漏
Root canal filling sealer
Calcium phosphate cement
Liquidity
Setting time
Antimicrobial activity
Dye microleakage
