摘要
目的 :观察体外应用羟基喜树碱 (HCPT)、平阳霉素 (PYM )、顺铂 (DDP)三种药物单用和两两联合应用对人舌癌Tca8113细胞的生长抑制作用。方法 :采用MTT法、细胞计数法、软琼脂克隆形成法及HE染色法 ,研究用药前后细胞增殖活性及形态的变化。结果 :⑴三种药单用及两药合用对细胞均有很好的抑制作用 ,且以HCPT和PYM合用最强。⑵HCPT和PYM单用和合用可使细胞的群体倍增时间显著延长 ,克隆形成率显著降低 ,形态发生明显的变化。结论
objective: To study the effects of hydroxycampothecin(HCPT), pingyangmycin(PYM), cis platinum (DDP) or their combination on the proliferation of human tongue carcinoma Tca8113 cells. Methods: MTT assay, cell counting,clonogenic assay and HE staining were used to study the effects of HCPT, PYM, DDP or their combination on the proliferation of human tongue carcinoma Tca8113 cells. Results: ①Synergistic inhibiton was observed when HCPT at the concentration (ng/ml) of 10～100 combined with PYM at 32～320 or DDP at 32～320, antagonistic effect was observed when PYM at 32～320 combined with DDP at 32～320; ②After treatment with PYM, HCPT or PYM plus HCPT at IC 30 the clonogenecity (%) of the cells was 15.2, 11.6 and 4.1 respectively, while that of untreated cells 31.7. Conclusion: The combination of HCPT with PYM may be synergistic in the inhibition of tongue carcinoma cell proliferation.
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
2003年第1期54-56,共3页
Journal of Practical Stomatology
