摘要
目的探讨3种大肠杆菌不耐热肠毒素突变体在辅佐幽门螺杆菌候选疫苗尿素酶B亚单位(rUreB)中的佐剂效应。方法各组Balb/c小鼠分别用PBS、rUreB、rUreB+LTK63、rUreB+LTR72、rUreB+LTKR及rUreB+CT进行4次口服免疫。ELISA检测胃、肠、气管冲洗液sIgA以及血清IgG亚类(IgG1,IgG2a);RT-PCR差异显示T淋巴细胞IFN-γ、IL-4 mRNA;ELISPOT检测肠派伊尔氏结IgA、IgG抗体分泌细胞。结果①各rUREB加突变体佐剂组在胃、肠、气管的sIgA和血清IgG1、IgG2a水平显著高于PBS组和单独rUreB组(P<0.01);②抗原刺激后取自各rUreB加突变体佐剂组T淋巴细胞表达的IFN-γ、IL-4 mRNA显著高于PBS组和rUreB组(P<0.01);③各rUreB加突变体佐剂组小肠派伊尔氏结抗体分泌细胞数都显著高于PBS组和单独rUreB组(P<0.01)。结论3种突变体都能辅佐rUreB在小鼠上产生特异的抗rUreB的抗体,且3种突变体诱导的免疫应答可能都是Th1/Th2型。LTR72的佐剂效应强于LTK63及LTKR。LTKR无毒,其稳定性高于LTR72,佐剂活性高于LTK63,是一个有希望的新型黏膜免疫佐剂。
Objective To investigate the ajuvanticifies of three heat-labile enterotoxin （LT） mutants in assisting Helicobacter pylori candidate vaccine rUreB. Methods Mice were orally vaccinated with PBS, rUreB, rUreB ＋ LTK63, rUreB ＋ LTR72, rUreB ＋ LTKR, and rUreB ＋ cr respectively for four times. ELISA was used to assay secretory IgA （in stomach, intestine, and trachea） and serum IgG subtypes （IgGl and IgG2a）. RT-PCR was used to test the mRNA levels of IFN-γ and IL-4 in lymphocytes. ELISPOT was used to count the antibody-secreting cells of intestine Peyer＇ s patchs. Results In LT mutant groups, the titers of sIgAs and sera IgG subtypes, the levels of IFN-γ and IL- 4 mRNA after stimulated by rUreB, and the antibedy-secreting cells of intestine payer＇s patchs were significantly higher than those of rUreB group and PBS group. Conclusion The three mutants can assist rUreB to elicit specific antibodies and induce Th1/Th2 balanced immune responses in mice. The adjuvanticity of LTR72 is significantly higher than that of LTK63 and LTKR. LTKR, a non-toxic mutant with higher stability than LTR72 and hie, her mucesal immune adjuvanticity than LTK63, is a new promising mucesal immune adjuvant.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2007年第6期640-644,共5页
Immunological Journal
基金
重庆市自然科学基金资助项目(20048716)
