摘要
目的:研究PPARα/γ双激动剂GCP-02对细胞葡萄糖利用、前脂肪细胞分化和细胞活力的影响。方法:以罗格列酮为对照,观察GCP-02对HepG2细胞和WB-F344细胞活力的影响,对HepG2细胞葡萄糖转运、葡萄糖消耗以及对3T3-L1细胞脂肪分化的影响。结果:在1×10-5mol/L浓度下,GCP-02降低WB-F344细胞活力,对HepG2细胞活力无影响;在不影响细胞活力浓度下,GCP-02增加HepG2细胞葡萄糖消耗,但不影响其葡萄糖转运;GCP-02增加3T3-L1细胞的脂肪生成。结论:GCP-02增加HepG2细胞的葡萄糖消耗,促进3T3-L1前脂肪细胞分化,降低WB-F344细胞活力。
Objective：To investigate the effects of GCP-02,a PPARα/γ dual agonist,on glucose utilization,pre-adipocyte differe-ntiation and cell viability in cultured cells. Methods：GCP-02 was tested for its effects on glucose transportation and consumption in HepG2 cells,on adipocyte differentiation in 3T3-L1 cells,respectively. Viability of HepG2 and WB-F344 cells was also investigated. Results：Viability of WB-F344 cells originated from rat was inhibited while that of HepG2 was not influenced by GCP-02 at the concentration of 10^-5 mol/L. GCP-02 increased glucose consumption but not glucose transportation in HepG2 cells. It promoted oil production at the concentrations with no influence on cell viability in 3T3-L1 cells. Conclusion：GCP-02 increased glucose consumption in HepG2 cells,promoted 3T3-L1 pre-adipocyte differentiation,suppressed viability in WB-F344 cells.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2007年第9期916-919,980,共5页
Journal of Nanjing Medical University(Natural Sciences)
基金
国家自然科学基金资助(30371672)
北京市自然科学基金资助(7042042)
