摘要
目的 研究维生素 (A、B、C、D、E、K各族 )对前脂肪细胞增殖和分化的作用。方法 培养人前脂肪细胞 ,在其生长的不同阶段分别加入各种维生素 ,观察其对细胞的增殖、分化过程中的磷酸甘油脱氢酶 (GPDH)和细胞内脂肪积聚的作用。结果 维生素A对人前脂肪细胞增殖和分化均有抑制作用 ,维生素B6 对人前脂肪细胞的分化有促进作用 ,维生素C对人前脂肪细胞增殖和分化均有明显的促进作用 ,维生素D3 对人前脂肪细胞的分化有抑制作用 ,维生素E对人前脂肪细胞的分化有较强的抑制作用 ,维生素K1对人前脂肪细胞分化有极强的刺激作用 ,维生素K3 对人前脂肪细胞的增殖和分化均有抑制作用。未发现其他维生素对人前脂肪细胞的明显作用。
ObjectiveTo study the effects of vitamins on human pr eadipocyte proliferation and differentiation. MethodsVit amins (A, B, C, D, E and K families) were added to the culture media of human p readipocytes, then the proliferation of cells, the expression of GPDH and lipid droplet accumulation in the cytoplasm were recorded. ResultsVitamin A inhibited human preadipocyte proliferation and differentiation. Vi tamin C enhanced differentiation and proliferation. Vitamin B 6 stimulated diff erentiation. vitamin D 3 inhibited differentiation. Vitamin E had a strong inhi bitory effect on human preadipocyte differentiation. Vitamin K 1 had an unexpec tedly great stimulatory effect on human preadipocyte differentiation. Vitamin K 3 inhibited both human preadipocyte proliferation and differentiation. Other v itamins tested showed no noticeable effects. ConclusionT hese data are advisory to our balanced daily intake of vitamins.
出处
《中华医学美学美容杂志》
2003年第6期356-359,共4页
Chinese Journal of Medical Aesthetics and Cosmetology
